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The essential role of complement in antibody-mediated resistance to Salmonella.

Published version
Peer-reviewed

Type

Article

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Authors

Rossi, Omar 
Coward, Chris 
Goh, Yun Shan 
Claassens, Jill WC 
MacLennan, Calman A 

Abstract

Vaccines can serve as essential tools to prevent bacterial diseases via the induction of long-lasting IgG responses. The efficacy of such vaccines depends on the effector mechanisms triggered by IgG. The complement system and Fc-gamma receptors (FcγRs) can potentially play a crucial role in IgG-mediated immunity against bacterial diseases. However, their relative importance in vivo is unclear, and has been the object of controversy and debate. In this brief study, we have used gene-targeted mice lacking either FcγRI, II, II and IV or the C3 complement component as well as a novel mouse strain lacking both C3 and FcγRs to conclusively show the essential role of complement in antibody-mediated host resistance to Salmonella enterica systemic infection. By comparing the effect of IgG2a antibodies against Salmonella O-antigen in gene-targeted mice, we demonstrate that the complement system is essential for the IgG-mediated reduction of bacterial numbers in the tissues.

Description

Keywords

Salmonella, in vivo, C3, FcγR, complement, infection, Animals, Bacterial Load, Complement Activation, Complement C3, Humans, Immunity, Humoral, Immunoglobulin G, Mice, Mice, Inbred C57BL, Mice, Knockout, O Antigens, Receptors, IgG, Salmonella Infections, Salmonella Vaccines, Salmonella enterica

Journal Title

Immunology

Conference Name

Journal ISSN

0019-2805
1365-2567

Volume Title

156

Publisher

Wiley
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/M000982/1)
Medical Research Council (G0001245)