The essential role of complement in antibody-mediated resistance to Salmonella.
Goh, Yun Shan
Claassens, Jill WC
MacLennan, Calman A
Verbeek, Sjef J
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Rossi, O., Coward, C., Goh, Y. S., Claassens, J. W., MacLennan, C. A., Verbeek, S. J., & Mastroeni, P. (2019). The essential role of complement in antibody-mediated resistance to Salmonella.. Immunology, 156 (1), 69-73. https://doi.org/10.1111/imm.13000
Vaccines can serve as essential tools to prevent bacterial diseases via the induction of long-lasting IgG responses. The efficacy of such vaccines depends on the effector mechanisms triggered by IgG. The complement system and Fc-gamma receptors (FcγRs) can potentially play a crucial role in IgG-mediated immunity against bacterial diseases. However, their relative importance in vivo is unclear, and has been the object of controversy and debate. In this brief study, we have used gene-targeted mice lacking either FcγRI, II, II and IV or the C3 complement component as well as a novel mouse strain lacking both C3 and FcγRs to conclusively show the essential role of complement in antibody-mediated host resistance to Salmonella enterica systemic infection. By comparing the effect of IgG2a antibodies against Salmonella O-antigen in gene-targeted mice, we demonstrate that the complement system is essential for the IgG-mediated reduction of bacterial numbers in the tissues.
Animals, Mice, Inbred C57BL, Mice, Knockout, Humans, Mice, Salmonella enterica, Salmonella Infections, O Antigens, Immunoglobulin G, Receptors, IgG, Salmonella Vaccines, Complement Activation, Complement C3, Immunity, Humoral, Bacterial Load
Biotechnology and Biological Sciences Research Council (BB/M000982/1)
Medical Research Council (G0001245)
External DOI: https://doi.org/10.1111/imm.13000
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285053
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/