The essential role of complement in antibody-mediated resistance to Salmonella.
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Authors
Rossi, Omar
Coward, Chris
Goh, Yun Shan
Claassens, Jill WC
MacLennan, Calman A
Verbeek, Sjef J
Publication Date
2019-01Journal Title
Immunology
ISSN
0019-2805
Publisher
Wiley
Volume
156
Issue
1
Pages
69-73
Language
eng
Type
Article
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Rossi, O., Coward, C., Goh, Y. S., Claassens, J. W., MacLennan, C. A., Verbeek, S. J., & Mastroeni, P. (2019). The essential role of complement in antibody-mediated resistance to Salmonella.. Immunology, 156 (1), 69-73. https://doi.org/10.1111/imm.13000
Abstract
Vaccines can serve as essential tools to prevent bacterial diseases via the induction of long-lasting IgG responses. The efficacy of such vaccines depends on the effector mechanisms triggered by IgG. The complement system and Fc-gamma receptors (FcγRs) can potentially play a crucial role in IgG-mediated immunity against bacterial diseases. However, their relative importance in vivo is unclear, and has been the object of controversy and debate. In this brief study, we have used gene-targeted mice lacking either FcγRI, II, II and IV or the C3 complement component as well as a novel mouse strain lacking both C3 and FcγRs to conclusively show the essential role of complement in antibody-mediated host resistance to Salmonella enterica systemic infection. By comparing the effect of IgG2a antibodies against Salmonella O-antigen in gene-targeted mice, we demonstrate that the complement system is essential for the IgG-mediated reduction of bacterial numbers in the tissues.
Keywords
Salmonella, in vivo, C3, FcγR, complement, infection, Animals, Bacterial Load, Complement Activation, Complement C3, Humans, Immunity, Humoral, Immunoglobulin G, Mice, Mice, Inbred C57BL, Mice, Knockout, O Antigens, Receptors, IgG, Salmonella Infections, Salmonella Vaccines, Salmonella enterica
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/M000982/1)
Medical Research Council (G0001245)
Identifiers
External DOI: https://doi.org/10.1111/imm.13000
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285053
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