You won't believe this old test … that does cheap single-cell mutation detection.
J Pathol Clin Res
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Köbel, M., Anglesio, M. S., & Brenton, J. (2018). You won't believe this old test … that does cheap single-cell mutation detection.. J Pathol Clin Res, 4 (3), 149-153. https://doi.org/10.1002/cjp2.108
Detecting mutations in single cells from cancer specimens is now a major area of translational research. In a recent article in this journal, Khalique et al validated an immunohistochemistry assay for ARID1A that reliably identifies loss of function mutations in single cells in tissue sections. This work exemplifies best practice for developing and orthogonally validating immunohistochemical assays to provide clearly interpretable mutational results with spatial context.
Humans, Genital Neoplasms, Female, DNA-Binding Proteins, Nuclear Proteins, Transcription Factors, Immunohistochemistry, Mutation, Female
Cancer Research UK (CB4150)
Cancer Research UK (C14303/A17197)
External DOI: https://doi.org/10.1002/cjp2.108
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285056
Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL: https://creativecommons.org/licenses/by-nc-nd/4.0/