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You won't believe this old test … that does cheap single-cell mutation detection.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Anglesio, Michael S  ORCID logo  https://orcid.org/0000-0003-1639-5003
Brenton, James D 

Abstract

Detecting mutations in single cells from cancer specimens is now a major area of translational research. In a recent article in this journal, Khalique et al validated an immunohistochemistry assay for ARID1A that reliably identifies loss of function mutations in single cells in tissue sections. This work exemplifies best practice for developing and orthogonally validating immunohistochemical assays to provide clearly interpretable mutational results with spatial context.

Description

Keywords

Female reproductive system, Immunohistochemistry, Mutation, Ovary, Testing, DNA-Binding Proteins, Female, Genital Neoplasms, Female, Humans, Immunohistochemistry, Mutation, Nuclear Proteins, Transcription Factors

Journal Title

J Pathol Clin Res

Conference Name

Journal ISSN

2056-4538
2056-4538

Volume Title

4

Publisher

Wiley
Sponsorship
Cancer Research UK (CB4150)
Cancer Research UK (C14303/A17197)