You won't believe this old test … that does cheap single-cell mutation detection.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Köbel, Martin https://orcid.org/0000-0002-6615-2037
Anglesio, Michael S https://orcid.org/0000-0003-1639-5003
Brenton, James D
Abstract
Detecting mutations in single cells from cancer specimens is now a major area of translational research. In a recent article in this journal, Khalique et al validated an immunohistochemistry assay for ARID1A that reliably identifies loss of function mutations in single cells in tissue sections. This work exemplifies best practice for developing and orthogonally validating immunohistochemical assays to provide clearly interpretable mutational results with spatial context.
Description
Keywords
Female reproductive system, Immunohistochemistry, Mutation, Ovary, Testing, DNA-Binding Proteins, Female, Genital Neoplasms, Female, Humans, Immunohistochemistry, Mutation, Nuclear Proteins, Transcription Factors
Journal Title
J Pathol Clin Res
Conference Name
Journal ISSN
2056-4538
2056-4538
2056-4538
Volume Title
4
Publisher
Wiley
Publisher DOI
Sponsorship
Cancer Research UK (CB4150)
Cancer Research UK (C14303/A17197)
Cancer Research UK (C14303/A17197)