A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses.
Canto Pastor, Alex
Yip, Wing Hin
Cold Spring Harbor Laboratory
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Wang, Z., Hardcastle, T., Canto Pastor, A., Yip, W. H., Tang, S., & Baulcombe, D. (2018). A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses.. Genes Dev, 32 (17-18), 1155-1160. https://doi.org/10.1101/gad.313601.118
Tomato Dicer-like2 (slDCL2) is a key component of resistance pathways against potato virus X (PVX) and tobacco mosaic virus (TMV). It is also required for production of endogenous small RNAs, including miR6026 and other noncanonical microRNAs (miRNAs). The slDCL2 mRNAs are targets of these slDCL2-dependent RNAs in a feedback loop that was disrupted by target mimic RNAs of miR6026. In lines expressing these RNAs, there was correspondingly enhanced resistance against PVX and TMV. These findings illustrate a novel miRNA pathway in plants and a crop protection strategy in which miRNA target mimicry elevates expression of defense-related mRNAs.
RNA Viruses, Lycopersicon esculentum, Disease Susceptibility, Ribonuclease III, MicroRNAs, Plant Diseases, Mutation, RNA, Small Untranslated, CRISPR-Cas Systems
European Research Council (340642)
International Balzan Prize Foundation (10957)
Royal Society (RP170001)
Royal Society (RP/EA/180018)
External DOI: https://doi.org/10.1101/gad.313601.118
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285087