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dc.contributor.authorCrozet, Pierre
dc.contributor.authorNavarro, Francisco J
dc.contributor.authorWillmund, Felix
dc.contributor.authorMehrshahi, Payam
dc.contributor.authorBakowski, Kamil
dc.contributor.authorLauersen, Kyle J
dc.contributor.authorPérez-Pérez, Maria-Esther
dc.contributor.authorAuroy, Pascaline
dc.contributor.authorGorchs Rovira, Aleix
dc.contributor.authorSauret-Gueto, Susana
dc.contributor.authorNiemeyer, Justus
dc.contributor.authorSpaniol, Benjamin
dc.contributor.authorTheis, Jasmine
dc.contributor.authorTrösch, Raphael
dc.contributor.authorWestrich, Lisa-Desiree
dc.contributor.authorVavitsas, Konstantinos
dc.contributor.authorBaier, Thomas
dc.contributor.authorHübner, Wolfgang
dc.contributor.authorde Carpentier, Felix
dc.contributor.authorCassarini, Mathieu
dc.contributor.authorDanon, Antoine
dc.contributor.authorHenri, Julien
dc.contributor.authorMarchand, Christophe H
dc.contributor.authorde Mia, Marcello
dc.contributor.authorSarkissian, Kevin
dc.contributor.authorBaulcombe, David
dc.contributor.authorPeltier, Gilles
dc.contributor.authorCrespo, José-Luis
dc.contributor.authorKruse, Olaf
dc.contributor.authorJensen, Poul-Erik
dc.contributor.authorSchroda, Michael
dc.contributor.authorSmith, Alison
dc.contributor.authorLemaire, Stéphane D
dc.date.accessioned2018-11-16T00:31:44Z
dc.date.available2018-11-16T00:31:44Z
dc.date.issued2018-09-21
dc.identifier.issn2161-5063
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285167
dc.description.abstractMicroalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherAmerican Chemical Society (ACS)
dc.subjectChlamydomonas reinhardtii
dc.subjectGreen Fluorescent Proteins
dc.subjectBiotechnology
dc.subjectPhotosynthesis
dc.subjectGene Expression
dc.subjectGenes, Reporter
dc.subjectPlasmids
dc.subjectPromoter Regions, Genetic
dc.subjectSynthetic Biology
dc.titleBirth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii.
dc.typeArticle
prism.endingPage2086
prism.issueIdentifier9
prism.publicationDate2018
prism.publicationNameACS Synth Biol
prism.startingPage2074
prism.volume7
dc.identifier.doi10.17863/CAM.32537
dcterms.dateAccepted2018-08-30
rioxxterms.versionofrecord10.1021/acssynbio.8b00251
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-09-05
dc.contributor.orcidCrozet, Pierre [0000-0001-5113-2407]
dc.contributor.orcidMehrshahi, Payam [0000-0002-7192-0942]
dc.contributor.orcidVavitsas, Konstantinos [0000-0002-6828-1000]
dc.contributor.orcidBaulcombe, David [0000-0003-0780-6878]
dc.contributor.orcidKruse, Olaf [0000-0001-9874-382X]
dc.contributor.orcidJensen, Poul-Erik [0000-0001-6524-7723]
dc.contributor.orcidSmith, Alison [0000-0001-6511-5704]
dc.identifier.eissn2161-5063
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/M018180/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L014130/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/D011043/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/I00680X/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/R021694/1)
cam.issuedOnline2018-08-30
rioxxterms.freetoread.startdate2019-09-05


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