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dc.contributor.authorTerrigno, Marco
dc.contributor.authorBertacchi, Michele
dc.contributor.authorPandolfini, Luca
dc.contributor.authorBaumgart, Mario
dc.contributor.authorCalvello, Mariantonietta
dc.contributor.authorCellerino, Alessandro
dc.contributor.authorStuder, Michele
dc.contributor.authorCremisi, Federico
dc.date.accessioned2018-11-22T00:30:48Z
dc.date.available2018-11-22T00:30:48Z
dc.date.issued2018-09-11
dc.identifier.issn2213-6711
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285596
dc.description.abstractThe morphogen FGF8 plays a pivotal role in neocortical area patterning through its inhibitory effect on COUP-TFI/Nr2f1 anterior expression, but its mechanism of action is poorly understood. We established an in vitro model of mouse embryonic stem cell corticogenesis in which COUP-TFI protein expression is inhibited by the activation of FGF8 in a time window corresponding to cortical area patterning. Interestingly, overexpression of the COUP-TFI 3′UTR reduces the inhibitory effect of FGF8 on COUP-TFI translation. FGF8 induces the expression of few miRNAs targeting COUP-TFI 3′UTR in silico. We found that the functional inhibition of miR-21 can effectively counteract the inhibitory effect of FGF8 in vitro and regulate COUP-TFI protein levels in vivo. Accordingly, miR-21 expression is complementary to COUP-TFI expression during corticogenesis. These data support a translational control of COUP-TFI gradient expression by FGF8 via miR-21 and contribute to our understanding of how regionalized expression is established during neocortical area mapping.
dc.publisherElsevier
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleThe microRNA miR-21 Is a Mediator of FGF8 Action on Cortical COUP-TFI Translation
dc.typeArticle
prism.endingPage769
prism.issueIdentifier3
prism.publicationDate2018
prism.publicationNameStem Cell Reports
prism.startingPage756
prism.volume11
dc.identifier.doi10.17863/CAM.32950
dcterms.dateAccepted2018-08-02
rioxxterms.versionofrecord10.1016/j.stemcr.2018.08.002
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.licenseref.startdate2018-09-11
dc.contributor.orcidPandolfini, Luca [0000-0003-1444-8167]
dc.identifier.eissn2213-6711
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-08-30


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International