Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR.
Duer, Melinda J
Shanahan, Catherine M
Chem Commun (Camb)
Royal Society of Chemistry (RSC)
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Chow, W. Y., Li, R., Goldberga, I., Reid, D. G., Rajan, R., Clark, J., Oschkinat, H., et al. (2018). Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR.. Chem Commun (Camb), 54 (89), 12570-12573. https://doi.org/10.1039/c8cc04960b
The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.
Is supplemented by: https://doi.org/10.17863/CAM.30578
Medical Research Council (MR/M01066X/1)
Biotechnology and Biological Sciences Research Council (BB/G021392/1)
External DOI: https://doi.org/10.1039/c8cc04960b
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285636