Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR.
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Authors
Li, Rui
Rajan, Rakesh
Clark, Jonathan
Oschkinat, Hartmut
Duer, Melinda J
Hayward, Robert
Shanahan, Catherine M
Publication Date
2018-11-06Journal Title
Chem Commun (Camb)
ISSN
1359-7345
Publisher
Royal Society of Chemistry (RSC)
Volume
54
Issue
89
Pages
12570-12573
Language
eng
Type
Article
Physical Medium
Print
Metadata
Show full item recordCitation
Chow, W. Y., Li, R., Goldberga, I., Reid, D. G., Rajan, R., Clark, J., Oschkinat, H., et al. (2018). Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR.. Chem Commun (Camb), 54 (89), 12570-12573. https://doi.org/10.1039/c8cc04960b
Abstract
The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.
Relationships
Is supplemented by: https://doi.org/10.17863/CAM.30578
Sponsorship
EPSRC (1652492)
Medical Research Council (MR/M01066X/1)
Biotechnology and Biological Sciences Research Council (BB/G021392/1)
Identifiers
External DOI: https://doi.org/10.1039/c8cc04960b
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285636
Rights
Licence:
http://www.rioxx.net/licenses/all-rights-reserved
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