Neuromesodermal progenitors are a conserved source of spinal cord with divergent growth dynamics.
van Oudenaarden, Alexander
Development (Cambridge, England)
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Attardi, A., Fulton, T., Florescu, M., Shah, G., Muresan, L., Lenz, M., Lancaster, C., et al. (2018). Neuromesodermal progenitors are a conserved source of spinal cord with divergent growth dynamics.. Development (Cambridge, England), 145 (21)https://doi.org/10.1242/dev.166728
During gastrulation, embryonic cells become specified into distinct germ layers. In mouse, this continues throughout somitogenesis from a population of bipotent stem cells called neuromesodermal progenitors (NMps). However, the degree of self-renewal associated with NMps in the fast-developing zebrafish embryo is unclear. With a genetic clone tracing method, we labelled early embryonic progenitors and find a strong clonal similarity between spinal cord and mesoderm tissues. We followed individual cell lineages by light-sheet imaging, revealing a common neuromesodermal lineage contribution to a subset of spinal cord tissue across the anterior-posterior body axis. An initial population subdivides at mid gastrula stages and is directly allocated to neural and mesodermal compartments during gastrulation. A second population in the tailbud undergoes delayed allocation to contribute to the neural and mesodermal compartment only at late somitogenesis. Cell tracking and retrospective cell fate assignment at late somitogenesis stages reveal these cells to be a collection of mono-fated progenitors. Our results suggest that NMps are a conserved population of bipotential progenitors, whose lineage varies in a species-specific manner due to vastly different rates of differentiation and growth.
Spinal Cord, Stem Cells, Tail, Mesoderm, Somites, Animals, Zebrafish, Cell Division, Organ Specificity, Cell Lineage, Body Patterning, Models, Biological, Gastrulation, Cell Tracking, Neural Stem Cells
WELLCOME TRUST (109408/Z/15/Z)
Wellcome Trust (099130/Z/12/Z)
External DOI: https://doi.org/10.1242/dev.166728
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285753
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/