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dc.contributor.authorVelsko, Irina M
dc.contributor.authorOvermyer, Katherine A
dc.contributor.authorSpeller, Camilla
dc.contributor.authorKlaus, Lauren
dc.contributor.authorCollins, Matthew
dc.contributor.authorLoe, Louise
dc.contributor.authorFrantz, Laurent AF
dc.contributor.authorSankaranarayanan, Krithivasan
dc.contributor.authorLewis, Cecil M
dc.contributor.authorMartinez, Juan Bautista Rodriguez
dc.contributor.authorChaves, Eros
dc.contributor.authorCoon, Joshua J
dc.contributor.authorLarson, Greger
dc.contributor.authorWarinner, Christina
dc.date.accessioned2018-11-23T00:33:23Z
dc.date.available2018-11-23T00:33:23Z
dc.date.issued2017
dc.identifier.issn1573-3882
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285857
dc.description.abstractINTRODUCTION: Dental calculus is a mineralized microbial dental plaque biofilm that forms throughout life by precipitation of salivary calcium salts. Successive cycles of dental plaque growth and calcification make it an unusually well-preserved, long-term record of host-microbial interaction in the archaeological record. Recent studies have confirmed the survival of authentic ancient DNA and proteins within historic and prehistoric dental calculus, making it a promising substrate for investigating oral microbiome evolution via direct measurement and comparison of modern and ancient specimens. OBJECTIVE: We present the first comprehensive characterization of the human dental calculus metabolome using a multi-platform approach. METHODS: Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) quantified 285 metabolites in modern and historic (200 years old) dental calculus, including metabolites of drug and dietary origin. A subset of historic samples was additionally analyzed by high-resolution gas chromatography-MS (GC-MS) and UPLC-MS/MS for further characterization of metabolites and lipids. Metabolite profiles of modern and historic calculus were compared to identify patterns of persistence and loss. RESULTS: Dipeptides, free amino acids, free nucleotides, and carbohydrates substantially decrease in abundance and ubiquity in archaeological samples, with some exceptions. Lipids generally persist, and saturated and mono-unsaturated medium and long chain fatty acids appear to be well-preserved, while metabolic derivatives related to oxidation and chemical degradation are found at higher levels in archaeological dental calculus than fresh samples. CONCLUSIONS: The results of this study indicate that certain metabolite classes have higher potential for recovery over long time scales and may serve as appropriate targets for oral microbiome evolutionary studies.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleThe dental calculus metabolome in modern and historic samples.
dc.typeArticle
prism.issueIdentifier11
prism.publicationDate2017
prism.publicationNameMetabolomics
prism.startingPage134
prism.volume13
dc.identifier.doi10.17863/CAM.33201
dcterms.dateAccepted2017-09-21
rioxxterms.versionofrecord10.1007/s11306-017-1270-3
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2017-01
dc.contributor.orcidCollins, Matthew [0000-0003-4226-5501]
dc.contributor.orcidWarinner, Christina [0000-0002-4528-5877]
dc.identifier.eissn1573-3890
rioxxterms.typeJournal Article/Review
cam.issuedOnline2017-10-03


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International