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dc.contributor.authorMarco Rius, Irene
dc.contributor.authorCheng, Tian
dc.contributor.authorGaunt, AP
dc.contributor.authorPatel, Saket
dc.contributor.authorKreis, Felix
dc.contributor.authorCapozzi, Andrea
dc.contributor.authorWright, Alan
dc.contributor.authorBrindle, Kevin
dc.contributor.authorOuari, Olivier
dc.contributor.authorComment, Arnaud
dc.description.abstractWhether for 13C magnetic resonance studies in chemistry, biochemistry or biomedicine, hyperpolarization methods based on dynamic nuclear polarization (DNP) have become ubiquitous. DNP requires a source of unpaired electrons, which are commonly added to the sample to be hyperpolarized in the form of stable free radicals. Once polarized, the presence of these radicals is unwanted. These radicals can be replaced by nonpersistent radicals created by photo-irradiation of pyruvic acid (PA), which are annihilated upon dissolution or thermalization in the solid state. However, since PA is readily metabolized by most cells, its presence may be undesirable for some metabolic studies. In addition, some 13C substrates are photo-sensitive and, therefore, may degrade during photo-generation of PA radical, which requires ultraviolet (UV) light. We show here that photoirradiation of phenylglyoxylic acid (PhGA) using visible light produces a non-persistent radical that, in principle, can be used to hyperpolarize any molecule. We compare radical yields in samples containing PA and PhGA upon photo-irradiation with broadband and narrowband UV-visible light sources. To demonstrate the suitability of PhGA as a radical precursor for DNP, we polarized the gluconeogenic probe 13C-dihydroxyacetone, which is UV-sensitive, using a commercial 3.35 T DNP polarizer and then injected this into a mouse and followed its metabolism in vivo.
dc.description.sponsorshipThis work is part of a project that has received funding from the European Union’s Horizon 2020 European Research Council (ERC Consolidator Grant) under grant agreement no. 682574 (ASSIMILES). Funding was also received from a Cancer Research UK Programme grant (17242) and from the CRUK-EPSRC Imaging Centre in Cambridge and Manchester (16465). F.K. and S.P. received funding from the European Union’s Horizon 2020 Research and Innovation Program under Marie Sklodowska-Curie grant agreement no. 642773 (EUROPOL). A. Capozzi received funding from the European Union’s Horizon 2020 Research and Innovation Program under Marie Sklodowska-Curie grant agreement no. 713683 (COFUNDfellowsDTU).
dc.publisherAmerican Chemical Society (ACS)
dc.rightsAttribution 4.0 International
dc.titlePhotogenerated Radical in Phenylglyoxylic Acid for in Vivo Hyperpolarized 13C MR with Photosensitive Metabolic Substrates
prism.publicationNameJournal of the American Chemical Society
dc.contributor.orcidKreis, Felix [0000-0003-3340-3861]
dc.contributor.orcidWright, Alan [0000-0002-4577-5681]
dc.contributor.orcidBrindle, Kevin [0000-0003-3883-6287]
dc.contributor.orcidComment, Arnaud [0000-0002-8484-3448]
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (642773)
pubs.funder-project-idCancer Research UK (CB4100)
pubs.funder-project-idCancer Research UK (C14303/A17197)
pubs.funder-project-idEuropean Research Council (682574)
cam.orpheus.successThu Jan 30 10:53:55 GMT 2020 - The item has an open VoR version.

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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International