DNA-directed immobilization of horseradish peroxidase onto porous SiO2 optical transducers.

Authors
Shtenberg, Giorgi 
Massad-Ivanir, Naama 
Engin, Sinem 
Sharon, Michal 

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Type
Article
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Abstract

Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-Pérot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-directed immobilization. Preliminary studies demonstrate high enzymatic activity levels of the immobilized horseradish peroxidase, while maintaining its specificity. The catalytic activity of the enzymes immobilized within the porous nanostructure is monitored in real time by reflective interferometric Fourier transform spectroscopy. We show that we can easily regenerate the surface for consecutive biosensing analysis by mild dehybridization conditions.

Publication Date
2012-08-08
Online Publication Date
2012-08-08
Acceptance Date
2012-07-11
Keywords
Porous Si, DNA immobilization, Enzyme, Nanostructure, Biosensor
Journal Title
Nanoscale Res Lett
Journal ISSN
1931-7573
1556-276X
Volume Title
7
Publisher
Springer Science and Business Media LLC