DNA-directed immobilization of horseradish peroxidase onto porous SiO2 optical transducers.
View / Open Files
Authors
Shtenberg, Giorgi
Massad-Ivanir, Naama
Engin, Sinem
Sharon, Michal
Segal, Ester
Publication Date
2012-08-08Journal Title
Nanoscale Res Lett
ISSN
1931-7573
Publisher
Springer Science and Business Media LLC
Volume
7
Issue
1
Pages
443
Language
eng
Type
Article
Physical Medium
Electronic
Metadata
Show full item recordCitation
Shtenberg, G., Massad-Ivanir, N., Engin, S., Sharon, M., Fruk, L., & Segal, E. (2012). DNA-directed immobilization of horseradish peroxidase onto porous SiO2 optical transducers.. Nanoscale Res Lett, 7 (1), 443. https://doi.org/10.1186/1556-276X-7-443
Abstract
Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-Pérot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-directed immobilization. Preliminary studies demonstrate high enzymatic activity levels of the immobilized horseradish peroxidase, while maintaining its specificity. The catalytic activity of the enzymes immobilized within the porous nanostructure is monitored in real time by reflective interferometric Fourier transform spectroscopy. We show that we can easily regenerate the surface for consecutive biosensing analysis by mild dehybridization conditions.
Identifiers
External DOI: https://doi.org/10.1186/1556-276X-7-443
This record's URL: https://www.repository.cam.ac.uk/handle/1810/286127
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.