Bimodal reflectance and fluorescence multispectral endoscopy based on spectrally resolving detector arrays.
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Authors
Luthman, A Siri
Ansel-Bollepalli, Laura
Publication Date
2018-10Journal Title
Journal of biomedical optics
ISSN
1083-3668
Publisher
S P I E - International Society for Optical Engineering
Volume
24
Issue
3
Pages
1-14
Language
eng
Type
Article
Physical Medium
Print
Metadata
Show full item recordCitation
Luthman, A. S., Waterhouse, D., Ansel-Bollepalli, L., Yoon, J., Gordon, G., Joseph, J., Di Pietro, M., et al. (2018). Bimodal reflectance and fluorescence multispectral endoscopy based on spectrally resolving detector arrays.. Journal of biomedical optics, 24 (3), 1-14. https://doi.org/10.1117/1.jbo.24.3.031009
Abstract
Emerging clinical interest in combining standard white light endoscopy with targeted near-infrared fluorescent
contrast agents for improved early cancer detection has created demand for multi-modal imaging endoscopes.
Here, we used two spectrally resolving detector arrays (SRDAs) to realize a bimodal endoscope capable of simultaneous
reflectance-based imaging in the visible spectral region and multiplexed fluorescence-based imaging in the
near-infrared. The visible SRDA was composed of 16 spectral bands, with peak wavelengths in the range 463-648 nm
and full width at half maximum (FWHM) between 9-26 nm. The near-infrared SRDA was composed of 25 spectral
bands, with peak wavelengths in the range 659-891 nm and FWHM 7-15 nm. The spectral endoscope design was based
on a ‘babyscope’ model using a commercially available imaging fiber bundle. We developed a spectral transmission
model to select optical components and provide reference endmembers for linear spectral unmixing of the recorded
image data. The technical characterization of the spectral endoscope is presented, including evaluation of the angular
field of view, barrel distortion, spatial resolution and spectral fidelity, which showed encouraging performance. An
agarose phantom tissue-mimicking containing oxygenated and deoxygenated blood with three fluorescent dyes was
then imaged. After spectral unmixing, the different chemical components of the phantom could be successfully identified
via majority decision with high signal to background ratio (>3). Imaging performance was further assessed in
an ex vivo porcine esophagus model. Our preliminary imaging results demonstrate the capability to simultaneously
resolve multiple biological components using a compact spectral endoscopy system.
Keywords
Fluorescent Dyes, Endoscopy, Spectrum Analysis, Fluorescence, Optical Imaging
Sponsorship
European Commission (630729)
Cancer Research UK (16267)
Cancer Research UK (16465)
Cancer Research UK (C14303_do not transfer)
Cancer Research UK (21102)
EPSRC (EP/R003599/1)
MRC (MC_PC_13059)
EPSRC (EP/N014588/1)
Identifiers
External DOI: https://doi.org/10.1117/1.jbo.24.3.031009
This record's URL: https://www.repository.cam.ac.uk/handle/1810/286209