Bimodal reflectance and fluorescence multispectral endoscopy based on spectrally resolving detector arrays.
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Authors
Luthman, A Siri
Waterhouse, Dale J
Ansel-Bollepalli, Laura
Gordon, George SD
di Pietro, Massimiliano
Januszewicz, Wladyslaw
Bohndiek, Sarah E
Publication Date
2018-10Journal Title
J Biomed Opt
ISSN
1083-3668
Publisher
SPIE-Intl Soc Optical Eng
Volume
24
Issue
3
Pages
1-14
Language
eng
Type
Article
Physical Medium
Print
Metadata
Show full item recordCitation
Luthman, A. S., Waterhouse, D. J., Ansel-Bollepalli, L., Yoon, J., Gordon, G. S., Joseph, J., di Pietro, M., et al. (2018). Bimodal reflectance and fluorescence multispectral endoscopy based on spectrally resolving detector arrays.. J Biomed Opt, 24 (3), 1-14. https://doi.org/10.1117/1.JBO.24.3.031009
Abstract
Emerging clinical interest in combining standard white light endoscopy with targeted near-infrared (NIR) fluorescent contrast agents for improved early cancer detection has created demand for multimodal imaging endoscopes. We used two spectrally resolving detector arrays (SRDAs) to realize a bimodal endoscope capable of simultaneous reflectance-based imaging in the visible spectral region and multiplexed fluorescence-based imaging in the NIR. The visible SRDA was composed of 16 spectral bands, with peak wavelengths in the range of 463 to 648 nm and full-width at half-maximum (FWHM) between 9 and 26 nm. The NIR SRDA was composed of 25 spectral bands, with peak wavelengths in the range 659 to 891 nm and FWHM 7 to 15 nm. The spectral endoscope design was based on a "babyscope" model using a commercially available imaging fiber bundle. We developed a spectral transmission model to select optical components and provide reference endmembers for linear spectral unmixing of the recorded image data. The technical characterization of the spectral endoscope is presented, including evaluation of the angular field-of-view, barrel distortion, spatial resolution and spectral fidelity, which showed encouraging performance. An agarose phantom containing oxygenated and deoxygenated blood with three fluorescent dyes was then imaged. After spectral unmixing, the different chemical components of the phantom could be successfully identified via majority decision with high signal-to-background ratio (>3). Imaging performance was further assessed in an ex vivo porcine esophagus model. Our preliminary imaging results demonstrate the capability to simultaneously resolve multiple biological components using a compact spectral endoscopy system.
Keywords
biomedical, endoscopy, fluorescence, hyperspectral, multiplexed, multispectral, spectrally resolving detector arrays, Endoscopy, Fluorescence, Fluorescent Dyes, Optical Imaging, Spectrum Analysis
Sponsorship
European Commission (630729)
Cancer Research Uk (None)
Cancer Research Uk (None)
Cancer Research UK (C14303/A17197)
Cancer Research UK (21102)
Engineering and Physical Sciences Research Council (EP/R003599/1)
Medical Research Council (MC_PC_13059)
Engineering and Physical Sciences Research Council (EP/N014588/1)
Identifiers
External DOI: https://doi.org/10.1117/1.JBO.24.3.031009
This record's URL: https://www.repository.cam.ac.uk/handle/1810/286209
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