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dc.contributor.authorLuthman, A Siri
dc.contributor.authorWaterhouse, Dale
dc.contributor.authorAnsel-Bollepalli, Laura
dc.contributor.authorYoon, Jonghee
dc.contributor.authorGordon, George
dc.contributor.authorJoseph, James
dc.contributor.authorDi Pietro, Massimiliano
dc.contributor.authorJanuszewicz, Wladyslaw
dc.contributor.authorBohndiek, Sarah
dc.date.accessioned2018-12-01T00:31:03Z
dc.date.available2018-12-01T00:31:03Z
dc.date.issued2018-10
dc.identifier.issn1083-3668
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/286209
dc.description.abstractEmerging clinical interest in combining standard white light endoscopy with targeted near-infrared (NIR) fluorescent contrast agents for improved early cancer detection has created demand for multimodal imaging endoscopes. We used two spectrally resolving detector arrays (SRDAs) to realize a bimodal endoscope capable of simultaneous reflectance-based imaging in the visible spectral region and multiplexed fluorescence-based imaging in the NIR. The visible SRDA was composed of 16 spectral bands, with peak wavelengths in the range of 463 to 648 nm and full-width at half-maximum (FWHM) between 9 and 26 nm. The NIR SRDA was composed of 25 spectral bands, with peak wavelengths in the range 659 to 891 nm and FWHM 7 to 15 nm. The spectral endoscope design was based on a "babyscope" model using a commercially available imaging fiber bundle. We developed a spectral transmission model to select optical components and provide reference endmembers for linear spectral unmixing of the recorded image data. The technical characterization of the spectral endoscope is presented, including evaluation of the angular field-of-view, barrel distortion, spatial resolution and spectral fidelity, which showed encouraging performance. An agarose phantom containing oxygenated and deoxygenated blood with three fluorescent dyes was then imaged. After spectral unmixing, the different chemical components of the phantom could be successfully identified via majority decision with high signal-to-background ratio (>3). Imaging performance was further assessed in an ex vivo porcine esophagus model. Our preliminary imaging results demonstrate the capability to simultaneously resolve multiple biological components using a compact spectral endoscopy system.
dc.format.mediumPrint
dc.languageeng
dc.publisherSPIE-Intl Soc Optical Eng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectFluorescent Dyes
dc.subjectEndoscopy
dc.subjectSpectrum Analysis
dc.subjectFluorescence
dc.subjectOptical Imaging
dc.titleBimodal reflectance and fluorescence multispectral endoscopy based on spectrally resolving detector arrays.
dc.typeArticle
prism.endingPage14
prism.issueIdentifier3
prism.publicationDate2018
prism.publicationNameJ Biomed Opt
prism.startingPage1
prism.volume24
dc.identifier.doi10.17863/CAM.33521
dcterms.dateAccepted2018-09-07
rioxxterms.versionofrecord10.1117/1.JBO.24.3.031009
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-10
dc.contributor.orcidWaterhouse, Dale [0000-0001-5530-6857]
dc.contributor.orcidYoon, Jonghee [0000-0003-3886-2792]
dc.contributor.orcidGordon, George [0000-0002-7333-5106]
dc.contributor.orcidJoseph, James [0000-0001-6270-2559]
dc.contributor.orcidDi Pietro, Massimiliano [0000-0003-4866-7026]
dc.contributor.orcidBohndiek, Sarah [0000-0003-0371-8635]
dc.identifier.eissn1560-2281
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Commission (630729)
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idCancer Research UK (C14303/A17197)
pubs.funder-project-idCancer Research UK (21102)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/R003599/1)
pubs.funder-project-idMedical Research Council (MC_PC_13059)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/N014588/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International