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A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane.

Published version
Peer-reviewed

Type

Article

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Authors

Gehrig, Peter 
Grossmann, Jonas 

Abstract

In terrestrial ecosystems most plant species live in mutualistic symbioses with nutrient-delivering arbuscular mycorrhizal (AM) fungi. Establishment of AM symbioses includes transient, intracellular formation of fungal feeding structures, the arbuscules. A plant-derived peri-arbuscular membrane (PAM) surrounds the arbuscules, mediating reciprocal nutrient exchange. Signaling at the PAM must be well coordinated to achieve this dynamic cellular intimacy. Here, we identify the PAM-specific Arbuscular Receptor-like Kinase 1 (ARK1) from maize and rice to condition sustained AM symbiosis. Mutation of rice ARK1 causes a significant reduction in vesicles, the fungal storage structures, and a concomitant reduction in overall root colonization by the AM fungus Rhizophagus irregularis. Arbuscules, although less frequent in the ark1 mutant, are morphologically normal. Co-cultivation with wild-type plants restores vesicle and spore formation, suggesting ARK1 function is required for the completion of the fungal life-cycle, thereby defining a functional stage, post arbuscule development.

Description

Keywords

Laser Capture Microdissection, Membrane Proteins, Membranes, Mutation, Mycorrhizae, Oryza, Plant Proteins, Promoter Regions, Genetic, Protein Serine-Threonine Kinases, Proteome, Receptors, Cell Surface, Symbiosis, Transcriptome, Zea mays

Journal Title

Nat Commun

Conference Name

Journal ISSN

2041-1723
2041-1723

Volume Title

9

Publisher

Springer Science and Business Media LLC
Sponsorship
European Commission (629887)
Biotechnology and Biological Sciences Research Council (BB/N008723/1)
Marie Curie FP7-PEOPLE-2013-IEF Grant number 629887 Gatsby Charitable Foundation RG60824 Isaac Newton Trust RG74108