Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development.
The Company of Biologists
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Boroviak, T., Stirparo, G., Dietmann, S., Hernando-Herraez, I., Mohammed, H., Reik, W., Smith, A., et al. (2018). Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development.. Development, 145 (21) https://doi.org/10.1242/dev.167833
The mouse embryo is the canonical model for mammalian preimplantation development. Recent advances in single cell profiling allow detailed analysis of embryogenesis in other eutherian species, including human, to distinguish conserved from divergent regulatory programs and signalling pathways in the rodent paradigm. Here, we identify and compare transcriptional features of human, marmoset and mouse embryos by single cell RNA-seq. Zygotic genome activation correlates with the presence of polycomb repressive complexes in all three species, while ribosome biogenesis emerges as a predominant attribute in primate embryos, supporting prolonged translation of maternally deposited RNAs. We find that transposable element expression signatures are species, stage and lineage specific. The pluripotency network in the primate epiblast lacks certain regulators that are operative in mouse, but encompasses WNT components and genes associated with trophoblast specification. Sequential activation of GATA6, SOX17 and GATA4 markers of primitive endoderm identity is conserved in primates. Unexpectedly, OTX2 is also associated with primitive endoderm specification in human and non-human primate blastocysts. Our cross-species analysis demarcates both conserved and primate-specific features of preimplantation development, and underscores the molecular adaptability of early mammalian embryogenesis.
Ribosomes, Germ Layers, Endoderm, Animals, Callithrix, Humans, Mice, RNA, Messenger, Gene Expression Profiling, Transcription, Genetic, Epigenesis, Genetic, Gene Expression Regulation, Developmental, Conserved Sequence, Embryonic Development, Otx Transcription Factors, Gene Regulatory Networks, Embryo, Mammalian, Single-Cell Analysis, Transcriptome
This work was supported by grants from the Biotechnology and Biological Sciences Research Council (BBSRC) UK (BB/M004023/1 (RG74277)), the Medical Research Council (MRC) UK (G1001028), and funding to the Cambridge Stem Cell Institute from the MRC and Wellcome Trust (097922/Z/11/Z, 203151/Z/16/Z). TB is a Wellcome Trust Sir Henry Dale Fellow. AS is an MRC Professor
Biotechnology and Biological Sciences Research Council (BB/M004023/1)
Wellcome Trust (097922/Z/11/Z)
Medical Research Council (G1001028)
Medical Research Council (MR/P00072X/1)
Wellcome Trust (203151/Z/16/Z)
External DOI: https://doi.org/10.1242/dev.167833
This record's URL: https://www.repository.cam.ac.uk/handle/1810/286383
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/