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dc.contributor.authorAshworth, Jennifer Cen
dc.contributor.authorMehr, Marcoen
dc.contributor.authorBuxton, Paul Gen
dc.contributor.authorBest, Serenaen
dc.contributor.authorCameron, Ruthen
dc.date.accessioned2018-12-07T00:31:03Z
dc.date.available2018-12-07T00:31:03Z
dc.date.issued2018-11-03en
dc.identifier.issn0957-4530
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/286396
dc.description.abstractDesign of cell-free scaffolds for endogenous cell recruitment requires an intimate knowledge of precise relationships between structure and biological function. Here, we use morphological analysis by Micro-CT to identify the key structural features necessary for periodontal ligament fibroblast recruitment into collagen scaffolds. By the combined use of time-lapse imaging and end-point invasion analysis, we distinguish the influences of pore size, pore wall alignment, and pore transport pathways (percolation diameter) on the individual cell migration and bulk invasion characteristics of these fibroblasts. Whereas maximising percolation diameter increased individual cell speed, elongation and directionality, and produced the most rapid bulk cell invasion, a pore size of 100 μm was found to be necessary to ensure an even distribution of cells across the scaffold cross-section. These results demonstrate that control of percolation diameter and pore size may be used respectively to tune the efficiency and uniformity of invasion through macroporous scaffolds. Crucially, however, these observations were subject to the condition of pore wall alignment, with low alignment in the direction of travel producing relatively low cell speeds and limited invasion in all cases. Pore wall alignment should therefore be carefully optimised in the design of scaffolds for cell recruitment, such as that required for periodontal ligament regeneration, as a key determining factor for cell movement.
dc.description.sponsorshipEngineering and Physical Sciences Research Council (EPSRC) grants EP/P505445/1, EP/J500380/1 and EP/N019938/1, Geistlich Pharma AG (of which PG Buxton and M Mehr are employees) and European Research Council (ERC) Advanced Grant 320598-3D-E
dc.format.mediumElectronicen
dc.languageengen
dc.publisherSpringer Nature
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectFibroblastsen
dc.subjectPeriodontal Ligamenten
dc.subjectHumansen
dc.subjectCollagenen
dc.subjectTissue Engineeringen
dc.subjectCell Movementen
dc.subjectTissue Scaffoldsen
dc.titleOptimising collagen scaffold architecture for enhanced periodontal ligament fibroblast migration.en
dc.typeArticle
prism.issueIdentifier11en
prism.publicationDate2018en
prism.publicationNameJournal of materials science. Materials in medicineen
prism.startingPage166
prism.volume29en
dc.identifier.doi10.17863/CAM.33708
dcterms.dateAccepted2018-10-17en
rioxxterms.versionofrecord10.1007/s10856-018-6175-9en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-11-03en
dc.contributor.orcidAshworth, Jennifer C [0000-0003-4189-8876]
dc.contributor.orcidBest, Serena [0000-0001-7866-8607]
dc.contributor.orcidCameron, Ruth [0000-0003-1573-4923]
dc.identifier.eissn1573-4838
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Research Council (320598)
pubs.funder-project-idEPSRC (EP/N019938/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International