Discovery of three RNA viruses using ant transcriptomic datasets.

Kleanthous, Eleni; Olendraite, Ingrida; Lukhovitskaya, Nina I; Firth, Andrew E

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Authors

Kleanthous, Eleni

Olendraite, Ingrida

Lukhovitskaya, Nina I

Firth, Andrew E

Publication Date

2019-02

Journal Title

Arch Virol

ISSN

0304-8608

Publisher

Springer Science and Business Media LLC

Volume

164

Issue

Pages

643-647

Language

eng

Type

Article

This Version

VoR

Physical Medium

Print-Electronic

Metadata

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Citation

Kleanthous, E., Olendraite, I., Lukhovitskaya, N. I., & Firth, A. E. (2019). Discovery of three RNA viruses using ant transcriptomic datasets.. Arch Virol, 164 (2), 643-647. https://doi.org/10.1007/s00705-018-4093-2

Abstract

Three novel RNA viruses, named Formica fusca virus 1 (GenBank accession no. MH477287), Lasius neglectus virus 2 (MH477288) and Myrmica scabrinodis virus 2 (MH477289), were discovered in ants collected in Cambridge, UK. The proposed virus names were given based on the hosts in which they were identified. The genome sequences were obtained using de novo transcriptome assembly of high-throughput RNA sequencing reads and confirmed by Sanger sequencing. Phylogenetic analysis showed that Formica fusca virus 1 grouped within the family Nyamiviridae, Lasius neglectus virus 2 grouped within the family Rhabdoviridae and Myrmica scabrinodis virus 2 belongs to the family Dicistroviridae. All three viruses are highly divergent from previously sequenced viruses.

Keywords

Animals, Ants, Genome, Viral, High-Throughput Nucleotide Sequencing, Insect Viruses, Open Reading Frames, Phylogeny, RNA Viruses, Transcriptome, Viral Proteins

Sponsorship

This work was supported by Wellcome Trust grant [106207] and European Research Council grant [646891] to A.E.F.

Funder references

Wellcome Trust (106207/Z/14/Z)

European Research Council (646891)

Identifiers

External DOI: https://doi.org/10.1007/s00705-018-4093-2

This record's URL: https://www.repository.cam.ac.uk/handle/1810/286411

Rights

Attribution 4.0 International

Licence URL: https://creativecommons.org/licenses/by/4.0/

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