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dc.contributor.authorCharles, Jermilia
dc.contributor.authorTangudu, Chandra S
dc.contributor.authorHurt, Stefanie L
dc.contributor.authorTumescheit, Charlotte
dc.contributor.authorFirth, Andrew
dc.contributor.authorGarcia-Rejon, Julian E
dc.contributor.authorMachain-Williams, Carlos
dc.contributor.authorBlitvich, Bradley J
dc.date.accessioned2018-12-08T00:30:33Z
dc.date.available2018-12-08T00:30:33Z
dc.date.issued2018-12
dc.identifier.issn0022-1317
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/286511
dc.description.abstractA metagenomics approach was used to detect novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico. A total of 1359 mosquitoes of 7 species and 5 genera (Aedes, Anopheles, Culex, Mansonia and Psorophora) were sorted into 37 pools, homogenized and inoculated onto monolayers of Aedes albopictus (C6/36) cells. A second blind passage was performed and then total RNA was extracted and analysed by RNA-seq. Two novel viruses, designated Uxmal virus and Mayapan virus, were identified. Uxmal virus was isolated from three pools of Aedes (Ochlerotatus) taeniorhynchus and phylogenetic data indicate that it should be classified within the recently proposed taxon Negevirus. Mayapan virus was recovered from two pools of Psorophora ferox and is most closely related to unclassified Nodaviridae-like viruses. Two recognized viruses were also detected: Culex flavivirus (family Flaviviridae) and Houston virus (family Mesoniviridae), with one and two isolates being recovered, respectively. The in vitro host ranges of all four viruses were determined by assessing their replicative abilities in cell lines of avian, human, monkey, hamster, murine, lepidopteran and mosquito (Aedes, Anopheles and Culex) origin, revealing that all viruses possess vertebrate replication-incompetent phenotypes. In conclusion, we report the isolation of both novel and recognized RNA viruses from mosquitoes collected in Mexico, and add to the growing plethora of viruses discovered recently through the use of metagenomics.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherMicrobiology Society
dc.subjectCell Line
dc.subjectAnimals
dc.subjectHumans
dc.subjectCulicidae
dc.subjectRNA Viruses
dc.subjectVirus Cultivation
dc.subjectSequence Analysis, DNA
dc.subjectSequence Analysis, RNA
dc.subjectBiodiversity
dc.subjectPhylogeny
dc.subjectMexico
dc.subjectMetagenomics
dc.subjectHost Specificity
dc.titleDetection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges.
dc.typeArticle
prism.endingPage1738
prism.issueIdentifier12
prism.publicationDate2018
prism.publicationNameJ Gen Virol
prism.startingPage1729
prism.volume99
dc.identifier.doi10.17863/CAM.33821
dcterms.dateAccepted2018-10-08
rioxxterms.versionofrecord10.1099/jgv.0.001165
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-12
dc.contributor.orcidFirth, Andrew [0000-0002-7986-9520]
dc.identifier.eissn1465-2099
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (106207/Z/14/Z)
pubs.funder-project-idEuropean Research Council (646891)


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