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dc.contributor.authorClift, Dean
dc.contributor.authorMcEwan, William
dc.contributor.authorLabzin, Larisa I
dc.contributor.authorKonieczny, Vera
dc.contributor.authorMogessie, Binyam
dc.contributor.authorJames, Leo C
dc.contributor.authorSchuh, Melina
dc.date.accessioned2018-12-13T00:32:53Z
dc.date.available2018-12-13T00:32:53Z
dc.date.issued2017-12-14
dc.identifier.issn0092-8674
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/286853
dc.description.abstractMethods for the targeted disruption of protein function have revolutionized science and greatly expedited the systematic characterization of genes. Two main approaches are currently used to disrupt protein function: DNA knockout and RNA interference, which act at the genome and mRNA level, respectively. A method that directly alters endogenous protein levels is currently not available. Here, we present Trim-Away, a technique to degrade endogenous proteins acutely in mammalian cells without prior modification of the genome or mRNA. Trim-Away harnesses the cellular protein degradation machinery to remove unmodified native proteins within minutes of application. This rapidity minimizes the risk that phenotypes are compensated and that secondary, non-specific defects accumulate over time. Because Trim-Away utilizes antibodies, it can be applied to a wide range of target proteins using off-the-shelf reagents. Trim-Away allows the study of protein function in diverse cell types, including non-dividing primary cells where genome- and RNA-targeting methods are limited.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectAntibodies
dc.subjectBiochemistry
dc.subjectProtein Transport
dc.subjectProteolysis
dc.titleA Method for the Acute and Rapid Degradation of Endogenous Proteins.
dc.typeArticle
prism.endingPage1706.e18
prism.issueIdentifier7
prism.publicationDate2017
prism.publicationNameCell
prism.startingPage1692
prism.volume171
dc.identifier.doi10.17863/CAM.34159
dcterms.dateAccepted2017-10-19
rioxxterms.versionofrecord10.1016/j.cell.2017.10.033
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2017-12
dc.contributor.orcidMcEwan, William [0000-0002-4408-0407]
dc.identifier.eissn1097-4172
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (206248/Z/17/Z)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International