Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi.
eLife Sciences Publications, Ltd
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Wang, M., Schäfer, M., Li, D., Halitschke, R., Dong, C., McGale, E., Paetz, C., et al. (2018). Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi.. Elife, 7 https://doi.org/10.7554/eLife.37093
High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.
Nicotiana attenuata, Rhizophagus irregularis, arbuscular mycorrhizal fungi, blumenol, colonization rate, ecology, high-throughput screening, plant biology, Biomarkers, Cyclohexanones, Genes, Plant, High-Throughput Screening Assays, Metabolomics, Mycorrhizae, Plant Leaves, Plant Shoots, RNA, Messenger, Stress, Physiological, Symbiosis, Time Factors, Tobacco
Biotechnology and Biological Sciences Research Council (BB/N008723/1)
Biotechnology and Biological Sciences Research Council (BB/P003419/1)
External DOI: https://doi.org/10.7554/eLife.37093
This record's URL: https://www.repository.cam.ac.uk/handle/1810/286935
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/
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