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dc.contributor.authorWang, Ming
dc.contributor.authorSchäfer, Martin
dc.contributor.authorLi, Dapeng
dc.contributor.authorHalitschke, Rayko
dc.contributor.authorDong, Chuanfu
dc.contributor.authorMcGale, Erica
dc.contributor.authorPaetz, Christian
dc.contributor.authorSong, Yuanyuan
dc.contributor.authorLi, Suhua
dc.contributor.authorDong, Junfu
dc.contributor.authorHeiling, Sven
dc.contributor.authorGroten, Karin
dc.contributor.authorFranken, Philipp
dc.contributor.authorBitterlich, Michael
dc.contributor.authorHarrison, Maria J
dc.contributor.authorPaszkowski, Uta
dc.contributor.authorBaldwin, Ian T
dc.date.accessioned2018-12-14T00:31:50Z
dc.date.available2018-12-14T00:31:50Z
dc.date.issued2018-08-28
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/286935
dc.description.abstractHigh-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.
dc.format.mediumElectronic
dc.languageeng
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMycorrhizae
dc.subjectTobacco
dc.subjectPlant Leaves
dc.subjectPlant Shoots
dc.subjectCyclohexanones
dc.subjectRNA, Messenger
dc.subjectSymbiosis
dc.subjectGenes, Plant
dc.subjectTime Factors
dc.subjectStress, Physiological
dc.subjectMetabolomics
dc.subjectHigh-Throughput Screening Assays
dc.subjectBiomarkers
dc.titleBlumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi.
dc.typeArticle
prism.publicationDate2018
prism.publicationNameElife
prism.volume7
dc.identifier.doi10.17863/CAM.34244
dcterms.dateAccepted2018-08-22
rioxxterms.versionofrecord10.7554/eLife.37093
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-08-28
dc.contributor.orcidSchäfer, Martin [0000-0002-4580-6337]
dc.contributor.orcidHalitschke, Rayko [0000-0002-1109-8782]
dc.contributor.orcidDong, Chuanfu [0000-0003-3043-7257]
dc.contributor.orcidMcGale, Erica [0000-0002-5996-4213]
dc.contributor.orcidFranken, Philipp [0000-0001-5710-4538]
dc.contributor.orcidBitterlich, Michael [0000-0002-3562-7327]
dc.contributor.orcidHarrison, Maria J [0000-0001-8716-1875]
dc.contributor.orcidPaszkowski, Uta [0000-0002-7279-7632]
dc.contributor.orcidBaldwin, Ian T [0000-0001-5371-2974]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/N008723/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/P003419/1)
cam.issuedOnline2018-08-28


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International