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dc.contributor.authorLopez, Ana
dc.contributor.authorFleming, Angeleen
dc.contributor.authorRubinsztein, David C
dc.date.accessioned2018-12-19T00:31:20Z
dc.date.available2018-12-19T00:31:20Z
dc.date.issued2018-10-24
dc.identifier.issn2046-2441
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287188
dc.description.abstractAutophagy is an intracellular clearance pathway that delivers cytoplasmic contents to the lysosome for degradation. It plays a critical role in maintaining protein homeostasis and providing nutrients under conditions where the cell is starved. It also helps to remove damaged organelles and misfolded or aggregated proteins. Thus, it is not surprising that defects in this pathway are associated with a variety of pathological conditions, such as neurodegeneration, cancer and infection. Pharmacological upregulation of autophagy is considered a promising therapeutic strategy for the treatment of neurodegenerative and infectious diseases. Studies in knockout mice have demonstrated that autophagy is essential for nervous system function, and data from invertebrate and vertebrate models suggest that the efficiency of autophagic processes generally declines with age. However, much of our understanding of the intracellular regulation of autophagy comes from in vitro studies, and there is a paucity of knowledge about how this process is regulated within different tissues and during the processes of ageing and disease. Here, we review the available tools to probe these questions in vivo within vertebrate model systems. We discuss how these tools have been used to date and consider future avenues of research.
dc.format.mediumElectronic
dc.languageeng
dc.publisherThe Royal Society
dc.subjectCell Line
dc.subjectLysosomes
dc.subjectAnimals
dc.subjectVertebrates
dc.subjectHumans
dc.subjectCommunicable Diseases
dc.subjectNeoplasms
dc.subjectNeurodegenerative Diseases
dc.subjectGreen Fluorescent Proteins
dc.subjectAge Factors
dc.subjectModels, Biological
dc.subjectAutophagy
dc.subjectAutophagy-Related Protein 8 Family
dc.titleSeeing is believing: methods to monitor vertebrate autophagy in vivo.
dc.typeArticle
prism.issueIdentifier10
prism.publicationDate2018
prism.publicationNameOpen Biol
prism.volume8
dc.identifier.doi10.17863/CAM.34497
dcterms.dateAccepted2018-09-25
rioxxterms.versionofrecord10.1098/rsob.180106
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-10-24
dc.contributor.orcidLopez, Ana [0000-0001-8105-7479]
dc.contributor.orcidFleming, Angeleen [0000-0003-3721-7126]
dc.contributor.orcidRubinsztein, David C [0000-0001-5002-5263]
dc.identifier.eissn2046-2441
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-10-24
rioxxterms.freetoread.startdate2019-10-24


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