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dc.contributor.authorHammond, Timothy R
dc.contributor.authorDufort, Connor
dc.contributor.authorDissing-Olesen, Lasse
dc.contributor.authorGiera, Stefanie
dc.contributor.authorYoung, Adam
dc.contributor.authorWysoker, Alec
dc.contributor.authorWalker, Alec J
dc.contributor.authorGergits, Frederick
dc.contributor.authorSegel, Michael
dc.contributor.authorNemesh, James
dc.contributor.authorMarsh, Samuel E
dc.contributor.authorSaunders, Arpiar
dc.contributor.authorMacosko, Evan
dc.contributor.authorGinhoux, Florent
dc.contributor.authorChen, Jinmiao
dc.contributor.authorFranklin, Robin JM
dc.contributor.authorPiao, Xianhua
dc.contributor.authorMcCarroll, Steven A
dc.contributor.authorStevens, Beth
dc.date.accessioned2018-12-20T00:31:00Z
dc.date.available2018-12-20T00:31:00Z
dc.date.issued2019-01-15
dc.identifier.issn1074-7613
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287230
dc.description.abstractMicroglia, the resident immune cells of the brain, rapidly change states in response to their environment, but we lack molecular and functional signatures of different microglial populations. Here, we analyzed the RNA expression patterns of more than 76,000 individual microglia in mice during development, in old age, and after brain injury. Our analysis uncovered at least nine transcriptionally distinct microglial states, which expressed unique sets of genes and were localized in the brain using specific markers. The greatest microglial heterogeneity was found at young ages; however, several states-including chemokine-enriched inflammatory microglia-persisted throughout the lifespan or increased in the aged brain. Multiple reactive microglial subtypes were also found following demyelinating injury in mice, at least one of which was also found in human multiple sclerosis lesions. These distinct microglia signatures can be used to better understand microglia function and to identify and manipulate specific subpopulations in health and disease.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherElsevier BV
dc.subjectBrain
dc.subjectMicroglia
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectHumans
dc.subjectMice
dc.subjectMultiple Sclerosis
dc.subjectBrain Injuries
dc.subjectDemyelinating Diseases
dc.subjectSequence Analysis, RNA
dc.subjectAdaptation, Physiological
dc.subjectCell Differentiation
dc.subjectAging
dc.subjectLongevity
dc.subjectSingle-Cell Analysis
dc.titleSingle-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State Changes.
dc.typeArticle
prism.endingPage271.e6
prism.issueIdentifier1
prism.publicationDate2019
prism.publicationNameImmunity
prism.startingPage253
prism.volume50
dc.identifier.doi10.17863/CAM.34537
dcterms.dateAccepted2018-11-02
rioxxterms.versionofrecord10.1016/j.immuni.2018.11.004
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-01
dc.contributor.orcidFranklin, Robin [0000-0001-6522-2104]
dc.identifier.eissn1097-4180
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MC_PC_12009)
cam.issuedOnline2018-11-21
rioxxterms.freetoread.startdate2019-11-21


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