Propensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites.
The Journal of general virology
Society for General Microbiology
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Stewart, H., Olspert, A., Butt, B., & Firth, A. (2019). Propensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites.. The Journal of general virology, 100 (2), 199-205. https://doi.org/10.1099/jgv.0.001189
The substitution rates of viral polymerases have been studied extensively. However less is known about the tendency of these enzymes to “slip” during RNA synthesis to produce progeny RNAs with nucleotide insertions or deletions. We recently described the functional utilisation of programmed polymerase slippage in family Potyviridae. This slippage results in either an insertion or a substitution, depending on whether or not the RNA duplex realigns following the insertion. In this study we investigated whether this phenomenon is a conserved feature of superfamily I viral RdRps by inserting a range of potyvirus-derived slip-prone sequences into a picornavirus, Theiler’s murine encephalomyelitis virus (TMEV). Deep sequencing analysis of viral transcripts indicates that the TMEV polymerase “slips” at U6–7 and A6–7 sequences to insert additional nucleotides. Such sequences are under-represented within picornaviral genomes, suggesting that slip-prone sequences create a fitness cost. Nonetheless, the TMEV insertional and substitutional spectrum differed from that previously determined for the potyvirus polymerase.
Potyvirus, Theilovirus, RNA, Viral, Mutagenesis, Insertional, Transcription, Genetic, High-Throughput Nucleotide Sequencing, RNA-Dependent RNA Polymerase
WELLCOME TRUST (106207/Z/14/Z)
European Commission Horizon 2020 (H2020) ERC (646891)
External DOI: https://doi.org/10.1099/jgv.0.001189
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287388
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/