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dc.contributor.authorStewart, Hazelen
dc.contributor.authorOlspert, Allanen
dc.contributor.authorButt, Benjaminen
dc.contributor.authorFirth, Andrewen
dc.date.accessioned2018-12-22T00:31:02Z
dc.date.available2018-12-22T00:31:02Z
dc.date.issued2019-02en
dc.identifier.issn0022-1317
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287388
dc.description.abstractThe substitution rates of viral polymerases have been studied extensively. However less is known about the tendency of these enzymes to “slip” during RNA synthesis to produce progeny RNAs with nucleotide insertions or deletions. We recently described the functional utilisation of programmed polymerase slippage in family Potyviridae. This slippage results in either an insertion or a substitution, depending on whether or not the RNA duplex realigns following the insertion. In this study we investigated whether this phenomenon is a conserved feature of superfamily I viral RdRps by inserting a range of potyvirus-derived slip-prone sequences into a picornavirus, Theiler’s murine encephalomyelitis virus (TMEV). Deep sequencing analysis of viral transcripts indicates that the TMEV polymerase “slips” at U6–7 and A6–7 sequences to insert additional nucleotides. Such sequences are under-represented within picornaviral genomes, suggesting that slip-prone sequences create a fitness cost. Nonetheless, the TMEV insertional and substitutional spectrum differed from that previously determined for the potyvirus polymerase.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherSociety for General Microbiology
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPotyvirusen
dc.subjectTheilovirusen
dc.subjectRNA, Viralen
dc.subjectMutagenesis, Insertionalen
dc.subjectTranscription, Geneticen
dc.subjectHigh-Throughput Nucleotide Sequencingen
dc.subjectRNA-Dependent RNA Polymeraseen
dc.titlePropensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites.en
dc.typeArticle
prism.endingPage205
prism.issueIdentifier2en
prism.publicationDate2019en
prism.publicationNameThe Journal of general virologyen
prism.startingPage199
prism.volume100en
dc.identifier.doi10.17863/CAM.34692
dcterms.dateAccepted2018-11-12en
rioxxterms.versionofrecord10.1099/jgv.0.001189en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-02en
dc.contributor.orcidOlspert, Allan [0000-0002-2990-3786]
dc.contributor.orcidButt, Benjamin [0000-0001-6718-0470]
dc.contributor.orcidFirth, Andrew [0000-0002-7986-9520]
dc.identifier.eissn1465-2099
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWELLCOME TRUST (106207/Z/14/Z)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) ERC (646891)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International