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dc.contributor.authorStewart, Hazel
dc.contributor.authorOlspert, Allan
dc.contributor.authorButt, Benjamin
dc.contributor.authorFirth, Andrew
dc.date.accessioned2018-12-22T00:31:02Z
dc.date.available2018-12-22T00:31:02Z
dc.date.issued2019-02
dc.identifier.issn0022-1317
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287388
dc.description.abstractThe substitution rates of viral polymerases have been studied extensively. However less is known about the tendency of these enzymes to 'slip' during RNA synthesis to produce progeny RNAs with nucleotide insertions or deletions. We recently described the functional utilization of programmed polymerase slippage in the family Potyviridae. This slippage results in either an insertion or a substitution, depending on whether the RNA duplex realigns following the insertion. In this study we investigated whether this phenomenon is a conserved feature of superfamily I viral RdRps, by inserting a range of potyvirus-derived slip-prone sequences into a picornavirus, Theiler's murine encephalomyelitis virus (TMEV). Deep-sequencing analysis of viral transcripts indicates that the TMEV polymerase 'slips' at the sequences U6-7 and A6-7 to insert additional nucleotides. Such sequences are under-represented within picornaviral genomes, suggesting that slip-prone sequences create a fitness cost. Nonetheless, the TMEV insertional and substitutional spectrum differed from that previously determined for the potyvirus polymerase.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherMicrobiology Society
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPotyvirus
dc.subjectTheilovirus
dc.subjectRNA, Viral
dc.subjectMutagenesis, Insertional
dc.subjectTranscription, Genetic
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.subjectRNA-Dependent RNA Polymerase
dc.titlePropensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites.
dc.typeArticle
prism.endingPage205
prism.issueIdentifier2
prism.publicationDate2019
prism.publicationNameJ Gen Virol
prism.startingPage199
prism.volume100
dc.identifier.doi10.17863/CAM.34692
dcterms.dateAccepted2018-11-12
rioxxterms.versionofrecord10.1099/jgv.0.001189
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-02
dc.contributor.orcidStewart, Hazel [0000-0003-1438-1089]
dc.contributor.orcidButt, Benjamin [0000-0001-6718-0470]
dc.contributor.orcidFirth, Andrew [0000-0002-7986-9520]
dc.identifier.eissn1465-2099
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (106207/Z/14/Z)
pubs.funder-project-idEuropean Research Council (646891)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International