Show simple item record

dc.contributor.authorHupalowska, Annaen
dc.contributor.authorJedrusik, Agnieszkaen
dc.contributor.authorZhu, Mengen
dc.contributor.authorBedford, Mark Ten
dc.contributor.authorGlover, Daviden
dc.contributor.authorZernicka-Goetz, Magdalenaen
dc.date.accessioned2018-12-22T00:31:28Z
dc.date.available2018-12-22T00:31:28Z
dc.date.issued2018-12en
dc.identifier.issn0092-8674
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287404
dc.description.abstractNuclear architecture has never been carefully examined during early mammalian development at the stages leading to establishment of the embryonic and extra-embryonic lineages. Heterogeneous activity of methyltransferase CARM1 during these stages results in differential methylation of histone H3R26 to modulate establishment of these two lineages. Here we show that CARM1 accumulates in nuclear granules at the 2- to 4-cell stage transition in the mouse embryo, the majority corresponding to paraspeckles. The paraspeckle components, Neat1 and its partner p54nrb, are required for CARM1’s association with paraspeckles and for H3R26 methylation. Conversely, CARM1 also influences paraspeckle organization. Depletion of Neat1 or p54nrb results in arrest at the 16- to 32-cell stage with elevated expression of transcription factor Cdx2, promoting differentiation into the extra-embryonic lineage. This developmental arrest occurs at an earlier stage than following CARM1 depletion indicating that paraspeckles act upstream of CARM1 but also have additional earlier roles in fate choice.
dc.format.mediumPrinten
dc.languageengen
dc.publisherElsevier
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBlastocysten
dc.subjectAnimalsen
dc.subjectMiceen
dc.subjectRNA-Binding Proteinsen
dc.subjectNuclear Matrix-Associated Proteinsen
dc.subjectCell Differentiationen
dc.subjectCell Lineageen
dc.subjectEmbryonic Developmenten
dc.subjectProtein-Arginine N-Methyltransferasesen
dc.subjectCell Cycle Checkpointsen
dc.subjectRNA, Long Noncodingen
dc.titleCARM1 and Paraspeckles Regulate Pre-implantation Mouse Embryo Development.en
dc.typeArticle
prism.endingPage1916.e13
prism.issueIdentifier7en
prism.publicationDate2018en
prism.publicationNameCellen
prism.startingPage1902
prism.volume175en
dc.identifier.doi10.17863/CAM.34708
dcterms.dateAccepted2018-11-16en
rioxxterms.versionofrecord10.1016/j.cell.2018.11.027en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-12en
dc.contributor.orcidZhu, Meng [0000-0001-6157-8840]
dc.contributor.orcidGlover, David [0000-0003-0956-0103]
dc.contributor.orcidZernicka-Goetz, Magdalena [0000-0002-7004-2471]
dc.identifier.eissn1097-4172
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Commission (657995)
pubs.funder-project-idWellcome Trust (098287/Z/12/Z)
pubs.funder-project-idWellcome Trust (207415/Z/17/Z)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International