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dc.contributor.authorHupalowska, Anna
dc.contributor.authorJedrusik, Agnieszka
dc.contributor.authorZhu, Meng
dc.contributor.authorBedford, Mark T
dc.contributor.authorGlover, David M
dc.contributor.authorZernicka-Goetz, Magdalena
dc.date.accessioned2018-12-22T00:31:28Z
dc.date.available2018-12-22T00:31:28Z
dc.date.issued2018-12-13
dc.identifier.issn0092-8674
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287404
dc.description.abstractNuclear architecture has never been carefully examined during early mammalian development at the stages leading to establishment of the embryonic and extra-embryonic lineages. Heterogeneous activity of the methyltransferase CARM1 during these stages results in differential methylation of histone H3R26 to modulate establishment of these two lineages. Here we show that CARM1 accumulates in nuclear granules at the 2- to 4-cell stage transition in the mouse embryo, with the majority corresponding to paraspeckles. The paraspeckle component Neat1 and its partner p54nrb are required for CARM1's association with paraspeckles and for H3R26 methylation. Conversely, CARM1 also influences paraspeckle organization. Depletion of Neat1 or p54nrb results in arrest at the 16- to 32-cell stage, with elevated expression of transcription factor Cdx2, promoting differentiation into the extra-embryonic lineage. This developmental arrest occurs at an earlier stage than following CARM1 depletion, indicating that paraspeckles act upstream of CARM1 but also have additional earlier roles in fate choice.
dc.format.mediumPrint
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBlastocyst
dc.subjectAnimals
dc.subjectMice
dc.subjectRNA-Binding Proteins
dc.subjectNuclear Matrix-Associated Proteins
dc.subjectCell Differentiation
dc.subjectCell Lineage
dc.subjectEmbryonic Development
dc.subjectProtein-Arginine N-Methyltransferases
dc.subjectCell Cycle Checkpoints
dc.subjectRNA, Long Noncoding
dc.titleCARM1 and Paraspeckles Regulate Pre-implantation Mouse Embryo Development.
dc.typeArticle
prism.endingPage1916.e13
prism.issueIdentifier7
prism.publicationDate2018
prism.publicationNameCell
prism.startingPage1902
prism.volume175
dc.identifier.doi10.17863/CAM.34708
dcterms.dateAccepted2018-11-16
rioxxterms.versionofrecord10.1016/j.cell.2018.11.027
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-12
dc.contributor.orcidZhu, Meng [0000-0001-6157-8840]
dc.contributor.orcidGlover, David [0000-0003-0956-0103]
dc.contributor.orcidZernicka-Goetz, Magdalena [0000-0002-7004-2471]
dc.identifier.eissn1097-4172
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (207415/Z/17/Z)
pubs.funder-project-idWellcome Trust (098287/Z/12/Z)
pubs.funder-project-idEuropean Commission (657995)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International