Differential viral accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration sites.
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Publication Date
2019-01Journal Title
Nature protocols
ISSN
1754-2189
Publisher
Springer Nature
Volume
14
Issue
1
Pages
153-170
Language
eng
Type
Article
This Version
AM
Physical Medium
Print
Metadata
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Timms, R., Tchasovnikarova, I., & Lehner, P. (2019). Differential viral accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration sites.. Nature protocols, 14 (1), 153-170. https://doi.org/10.1038/s41596-018-0087-5
Abstract
Alterations in chromatin structure play a major role in the epigenetic regulation of gene expression. Here, we describe a step-by-step protocol for differential viral accessibility (DIVA), a method for identifying changes in chromatin accessibility genome-wide. Commonly used methods for mapping accessible genomic loci have strong preferences toward detecting 'open' chromatin found at regulatory regions but are not well suited to studying chromatin accessibility in gene bodies and intergenic regions. DIVA overcomes this limitation, enabling a broader range of sites to be interrogated. Conceptually, DIVA is similar to ATAC-seq in that it relies on the integration of exogenous DNA into the genome to map accessible chromatin, except that chromatin architecture is probed through mapping integration sites of exogenous lentiviruses. An isogenic pair of cell lines are transduced with a lentiviral vector, followed by PCR amplification and Illumina sequencing of virus-genome junctions; the resulting sequences define a set of unique lentiviral integration sites, which are compared to determine whether genomic loci exhibit significantly altered accessibility between experimental and control cells. Experienced researchers will take 6 d to generate lentiviral stocks and transduce the target cells, a further 5 d to prepare the Illumina sequencing libraries and a few hours to perform the bioinformatic analysis.
Keywords
Chromatin, Humans, Lentivirus, DNA, Viral, Chromosome Mapping, Transduction, Genetic, Polymerase Chain Reaction, Virus Integration, Genomic Library, Genome, Human, Genetic Loci, High-Throughput Nucleotide Sequencing
Sponsorship
Wellcome Trust (101835/Z/13/Z)
WELLCOME TRUST (201387/Z/16/Z)
MRC (MR/R001405/1)
Identifiers
External DOI: https://doi.org/10.1038/s41596-018-0087-5
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287443
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