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dc.contributor.authorGatto, Laurent
dc.contributor.authorBreckels, Lisa M
dc.contributor.authorLilley, Kathryn
dc.date.accessioned2019-01-03T00:30:14Z
dc.date.available2019-01-03T00:30:14Z
dc.date.issued2019-02
dc.identifier.issn1367-5931
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287483
dc.description.abstractThe sub-cellular localisation of a protein is vital in defining its function, and a protein's mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions, including high profile mass spectrometry-based efforts. Here, we present a meta-analysis assessing and comparing the sub-cellular resolution of 29 such mass spectrometry-based spatial proteomics experiments using a newly developed tool termed QSep. Our goal is to provide a simple quantitative report of how well spatial proteomics resolve the sub-cellular niches they describe to inform and guide developers and users of such methods.
dc.description.sponsorshipThis work was supported by a BBSRC Strategic Longer and Larger grant (Award BB/L002817/1), a Wellcome Trust Technology Development Grant (Grant number 108441/Z/15/Z) and a BBSRC Tools and resources development grant (Award BB/N023129/1).
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCellular Structures
dc.subjectAnimals
dc.subjectHumans
dc.subjectProteins
dc.subjectProteomics
dc.subjectMass Spectrometry
dc.titleAssessing sub-cellular resolution in spatial proteomics experiments.
dc.typeArticle
prism.endingPage149
prism.publicationDate2019
prism.publicationNameCurr Opin Chem Biol
prism.startingPage123
prism.volume48
dc.identifier.doi10.17863/CAM.33543
dcterms.dateAccepted2018-11-19
rioxxterms.versionofrecord10.1016/j.cbpa.2018.11.015
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-02
dc.contributor.orcidGatto, Laurent [0000-0002-1520-2268]
dc.contributor.orcidLilley, Kathryn [0000-0003-0594-6543]
dc.identifier.eissn1879-0402
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L018497/1)
pubs.funder-project-idWellcome Trust (108441/Z/15/Z)
pubs.funder-project-idWellcome Trust (110170/Z/15/Z)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/K00137X/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/N023129/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L002817/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International