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The subcellular organisation of Saccharomyces cerevisiae.

Published version
Peer-reviewed

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Authors

Nightingale, Daniel Jh 
Geladaki, Aikaterini 
Breckels, Lisa M 
Oliver, Stephen G 
Lilley, Kathryn S 

Abstract

Subcellular protein localisation is essential for the mechanisms that govern cellular homeostasis. The ability to understand processes leading to this phenomenon will therefore enhance our understanding of cellular function. Here we review recent developments in this field with regard to mass spectrometry, fluorescence microscopy and computational prediction methods. We highlight relative strengths and limitations of current methodologies focussing particularly on studies in the yeast Saccharomyces cerevisiae. We further present the first cell-wide spatial proteome map of S. cerevisiae, generated using hyperLOPIT, a mass spectrometry-based protein correlation profiling technique. We compare protein subcellular localisation assignments from this map, with two published fluorescence microscopy studies and show that confidence in localisation assignment is attained using multiple orthogonal methods that provide complementary data.

Description

Keywords

Mass Spectrometry, Microscopy, Fluorescence, Proteomics, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins

Journal Title

Curr Opin Chem Biol

Conference Name

Journal ISSN

1367-5931
1879-0402

Volume Title

48

Publisher

Elsevier BV
Sponsorship
Wellcome Trust (108441/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/N023129/1)
Biotechnology and Biological Sciences Research Council (BB/K00137X/1)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/L002817/1)
BBSRC and Wellcome Trust