Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly.
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Authors
Iannucci, Alessio
Altmanová, Marie
Ciofi, Claudio
Pereira, Jorge C
Rehák, Ivan
Stanyon, Roscoe
Velenský, Petr
Rovatsos, Michail
Kratochvíl, Lukáš
Johnson Pokorná, Martina
Publication Date
2019Journal Title
Cytogenet Genome Res
ISSN
1424-8581
Publisher
S. Karger AG
Volume
157
Issue
1-2
Pages
123-131
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Iannucci, A., Altmanová, M., Ciofi, C., Ferguson-Smith, M., Pereira, J. C., Rehák, I., Stanyon, R., et al. (2019). Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly.. Cytogenet Genome Res, 157 (1-2), 123-131. https://doi.org/10.1159/000496171
Abstract
We developed new tools to build a high-quality chromosomal map of the Komodo dragon (Varanus komodoensis) available for cross-species phylogenomic analyses. First, we isolated chromosomes by flow sorting and determined the chromosome content of each flow karyotype peak by FISH. We then isolated additional Komodo dragon chromosomes by microdissection and amplified chromosome-specific DNA pools. The chromosome-specific DNA pools can be sequenced, assembled, and mapped by next-generation sequencing technology. The chromosome-specific paint probes can be used to investigate karyotype evolution through cross-species chromosome painting. Overall, the set of chromosome-specific DNA pools of V. komodoensis provides new tools for detailed phylogenomic analyses of Varanidae and squamates in general.
Keywords
Chromosomes, Animals, Lizards, DNA, DNA Probes, Chromosome Banding, Chromosome Painting, Chromosome Mapping, Female, High-Throughput Nucleotide Sequencing, Karyotype
Sponsorship
none
Identifiers
External DOI: https://doi.org/10.1159/000496171
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287990
Rights
Licence:
http://www.rioxx.net/licenses/all-rights-reserved
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