The Chick Caudolateral Epiblast Acts as a Permissive Niche for Generating Neuromesodermal Progenitor Behaviours.
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Publication Date
2018Journal Title
Cells Tissues Organs
ISSN
1422-6405
Publisher
S. Karger AG
Volume
205
Issue
5-6
Pages
320-330
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Baillie-Johnson, P., Voiculescu, O., Hayward, P., & Steventon, B. (2018). The Chick Caudolateral Epiblast Acts as a Permissive Niche for Generating Neuromesodermal Progenitor Behaviours.. Cells Tissues Organs, 205 (5-6), 320-330. https://doi.org/10.1159/000494769
Abstract
Neuromesodermal progenitors (NMps) are a population of bipotent progenitors that maintain competence to generate both spinal cord and paraxial mesoderm throughout the elongation of the posterior body axis. Recent studies have generated populations of NMp-like cells in culture, which have been shown to differentiate to both neural and mesodermal cell fates when transplanted into either mouse or chick embryos. Here, we aim to compare the potential of mouse embryonic stem (ES) cell-derived progenitor populations to generate NMp behaviour against both undifferentiated and differentiated populations. We define NMp behaviour as the ability of cells to: (i) contribute to a significant proportion of the anterior-posterior body axis, (ii) enter into both posterior neural and somitic compartments, and (iii) retain a proportion of the progenitor population within the posterior growth zone. We compare previously identified ES cell-derived NMp-like populations to undifferentiated mouse ES cells and find that they all display similar potentials to generate NMp behaviour in vivo. To assess whether this competence is lost upon further differentiation, we generated anterior and posterior embryonic cell types through the generation of 3D gastruloids and show that NMp competence is lost within the anterior (Brachyury-negative) portion of the gastruloid. Together this suggests that in vitro-derived NMp-like cells maintain an ability to contribute to multiple germ layers that is also present within pluripotent ES cells, rather than acquiring a neuromesodermal competent state through differentiation.
Keywords
Spinal Cord, Cell Line, Chick Embryo, Germ Layers, Mesoderm, Animals, Mice, Embryo Culture Techniques, Cell Differentiation, Cell Lineage, Body Patterning, Neural Stem Cells, Mouse Embryonic Stem Cells
Sponsorship
Wellcome Trust (109408) and (RCDF 088380/09/Z)
Engineering and Physical Sciences Research Council (EPSRC) Studentship (1359454).
Funder references
Wellcome Trust (109408/Z/15/Z)
Wellcome Trust (088380/Z/09/Z)
Identifiers
External DOI: https://doi.org/10.1159/000494769
This record's URL: https://www.repository.cam.ac.uk/handle/1810/288039
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL: https://creativecommons.org/licenses/by-nc-nd/4.0/
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