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dc.contributor.authorRoberts, Geoffrey P
dc.contributor.authorLarraufie, Pierre
dc.contributor.authorRichards, Paul
dc.contributor.authorKay, Richard G
dc.contributor.authorGalvin, Sam G
dc.contributor.authorMiedzybrodzka, Emily L
dc.contributor.authorLeiter, Andrew
dc.contributor.authorLi, H Joyce
dc.contributor.authorGlass, Leslie L
dc.contributor.authorMa, Marcella KL
dc.contributor.authorLam, Brian
dc.contributor.authorYeo, Giles SH
dc.contributor.authorScharfmann, Raphaël
dc.contributor.authorChiarugi, Davide
dc.contributor.authorHardwick, Richard H
dc.contributor.authorReimann, Frank
dc.contributor.authorGribble, Fiona M
dc.date.accessioned2019-01-16T00:31:56Z
dc.date.available2019-01-16T00:31:56Z
dc.date.issued2019-05
dc.identifier.issn0012-1797
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/288066
dc.description.abstractEnteroendocrine cells (EECs) produce hormones such as glucagon-like peptide 1 and peptide YY that regulate food absorption, insulin secretion, and appetite. Based on the success of glucagon-like peptide 1-based therapies for type 2 diabetes and obesity, EECs are themselves the focus of drug discovery programs to enhance gut hormone secretion. The aim of this study was to identify the transcriptome and peptidome of human EECs and to provide a cross-species comparison between humans and mice. By RNA sequencing of human EECs purified by flow cytometry after cell fixation and staining, we present a first transcriptomic analysis of human EEC populations and demonstrate a strong correlation with murine counterparts. RNA sequencing was deep enough to enable identification of low-abundance transcripts such as G-protein-coupled receptors and ion channels, revealing expression in human EECs of G-protein-coupled receptors previously found to play roles in postprandial nutrient detection. With liquid chromatography-tandem mass spectrometry, we profiled the gradients of peptide hormones along the human and mouse gut, including their sequences and posttranslational modifications. The transcriptomic and peptidomic profiles of human and mouse EECs and cross-species comparison will be valuable tools for drug discovery programs and for understanding human metabolism and the endocrine impacts of bariatric surgery.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherAmerican Diabetes Association
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectDiabetes Mellitus, Type 2
dc.subjectReceptors, G-Protein-Coupled
dc.subjectEnteroendocrine Cells
dc.subjectGlucagon-Like Peptide 1
dc.subjectTranscriptome
dc.titleComparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.
dc.typeArticle
prism.endingPage1072
prism.issueIdentifier5
prism.publicationDate2019
prism.publicationNameDiabetes
prism.startingPage1062
prism.volume68
dc.identifier.doi10.17863/CAM.35385
dcterms.dateAccepted2018-10-23
rioxxterms.versionofrecord10.2337/db18-0883
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-05
dc.contributor.orcidRoberts, Geoffrey P [0000-0001-9554-8404]
dc.contributor.orcidScharfmann, Raphaël [0000-0001-7619-337X]
dc.contributor.orcidGribble, Fiona M [0000-0002-4232-2898]
dc.identifier.eissn1939-327X
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MC_UU_12012/3)
pubs.funder-project-idWellcome Trust (106262/Z/14/Z)
pubs.funder-project-idWellcome Trust (100574/Z/12/Z)
pubs.funder-project-idMedical Research Council (MC_UU_12012/5)
pubs.funder-project-idEuropean Foundation for the Study of Diabetes (EFSD) (unknown)
pubs.funder-project-idRoyal College of Surgeons of England (unknown)
pubs.funder-project-idWellcome Trust (088357/Z/09/Z)
pubs.funder-project-idWellcome Trust (084210/Z/07/Z)
pubs.funder-project-idMedical Research Council (MC_UU_12012/1)
pubs.funder-project-idMedical Research Council (MR/M009041/1)
pubs.funder-project-idMedical Research Council (MR/M024873/1)
pubs.funder-project-idMRC (MC_UU_00014/3)
pubs.funder-project-idMRC (MC_UU_00014/5)
cam.issuedOnline2019-02-07
cam.orpheus.successTue Mar 31 10:42:38 BST 2020 - Embargo updated
rioxxterms.freetoread.startdate2019-05-31


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