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dc.contributor.authorSantiago, AC
dc.contributor.authorKhan, ZN
dc.contributor.authorMiguel, MC
dc.contributor.authorGironda, CC
dc.contributor.authorSoares-Costa, A
dc.contributor.authorPelá, VT
dc.contributor.authorLeite, AL
dc.contributor.authorEdwardson, JM
dc.contributor.authorBuzalaf, MAR
dc.contributor.authorHenrique-Silva, F
dc.date.accessioned2019-01-16T10:55:26Z
dc.date.available2019-01-16T10:55:26Z
dc.date.issued2017-08-01
dc.identifier.issn0022-0345
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/288076
dc.description.abstractCystatin B was recently identified as an acid-resistant protein in acquired enamel pellicle; it could therefore be included in oral products to protect against caries and erosion. However, human recombinant cystatin is very expensive, and alternatives to its use are necessary. Phytocystatins are reversible inhibitors of cysteine peptidases that are found naturally in plants. In plants, they have several biological and physiological functions, such as the regulation of endogenous processes, defense against pathogens, and response to abiotic stress. Previous studies performed by our research group have reported high inhibitory activity and potential agricultural and medical applications of several sugarcane cystatins, including CaneCPI-1, CaneCPI-2, CaneCPI-3, and CaneCPI-4. In the present study, we report the characterization of a novel sugarcane cystatin, named CaneCPI-5. This cystatin was efficiently expressed in Escherichia coli, and inhibitory assays demonstrated that it was a potent inhibitor of human cathepsins B, K, and L ( Ki = 6.87, 0.49, and 0.34 nM, respectively). The ability of CaneCPI-5 to bind to dental enamel was evaluated using atomic force microscopy. Its capacity to protect against initial enamel erosion was also tested in vitro via changes in surface hardness. CaneCPI-5 showed a very large force of interaction with enamel (e.g., compared with mucin and casein) and significantly reduced initial enamel erosion. These results suggest that the inclusion of CaneCPIs in dental products might confer protection against enamel erosion.
dc.description.sponsorshipThis research was supported by the São Paulo Research Foundation (FAPESP) (CEPID-CBME #98/14138-2) and by the Royal Society (Newton Advanced Fellowship #NA140459). A.C.S. is a recipient of a scholarship from the National Council for Scientific and Technological Development (CNPq). M.C.M. received a grant from the Coordination for the Improvement of Higher Education Personnel (CAPES). V.T.P. received a grant from the FAPESP. F.H.-S. and M.A.R.B. were recipients of a productivity scholarship from the CNPq.
dc.languageeng
dc.publisherSAGE Publications
dc.subjectatomic force microscopy
dc.subjectcysteine peptidase inhibitor
dc.subjectcysteine peptidases
dc.subjectdental erosion
dc.subjecthuman cathepsin
dc.subjectphytocystatin
dc.subjectAnimals
dc.subjectCathepsins
dc.subjectCattle
dc.subjectCystatins
dc.subjectDental Enamel
dc.subjectEscherichia coli
dc.subjectIn Vitro Techniques
dc.subjectIncisor
dc.subjectMicroscopy, Atomic Force
dc.subjectSaccharum
dc.subjectTooth Erosion
dc.titleA New Sugarcane Cystatin Strongly Binds to Dental Enamel and Reduces Erosion.
dc.typeArticle
prism.endingPage1057
prism.issueIdentifier9
prism.publicationDate2017
prism.publicationNameJournal of Dental Research
prism.startingPage1051
prism.volume96
dc.identifier.doi10.17863/CAM.35394
dcterms.dateAccepted2017-05-01
rioxxterms.versionofrecord10.1177/0022034517712981
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2017-08-01
dc.identifier.eissn1544-0591
rioxxterms.typeJournal Article/Review
cam.issuedOnline2017-06-12


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