ZNF445 is a primary regulator of genomic imprinting.
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Authors
Coluccio, Andrea
Thorball, Christian W
Planet, Evarist
Shi, Hui
Offner, Sandra
Turelli, Priscilla
Ferguson-Smith, Anne C
Trono, Didier
Publication Date
2019-01-01Journal Title
Genes Dev
ISSN
0890-9369
Publisher
Cold Spring Harbor Laboratory
Volume
33
Issue
1-2
Pages
49-54
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print
Metadata
Show full item recordCitation
Takahashi, N., Coluccio, A., Thorball, C. W., Planet, E., Shi, H., Offner, S., Turelli, P., et al. (2019). ZNF445 is a primary regulator of genomic imprinting.. Genes Dev, 33 (1-2), 49-54. https://doi.org/10.1101/gad.320069.118
Abstract
Genomic imprinting is an epigenetic process regulated by germline-derived DNA methylation, causing parental origin-specific monoallelic gene expression. Zinc finger protein 57 (ZFP57) is critical for maintenance of this epigenetic memory during post-fertilization reprogramming, yet incomplete penetrance of ZFP57 mutations in humans and mice suggests additional effectors. We reveal that ZNF445/ZFP445, which we trace to the origins of imprinting, binds imprinting control regions (ICRs) in mice and humans. In mice, ZFP445 and ZFP57 act together, maintaining all but one ICR in vivo, whereas earlier embryonic expression of ZNF445 and its intolerance to loss-of-function mutations indicate greater importance in the maintenance of human imprints.
Keywords
Cells, Cultured, Animals, Mice, Inbred C57BL, Humans, Mice, Transcription Factors, Repressor Proteins, DNA Methylation, Genomic Imprinting, Conserved Sequence, Kruppel-Like Transcription Factors, Embryonic Stem Cells, HEK293 Cells
Sponsorship
Medical Research Council (MR/J001597/1)
Wellcome Trust (095606/Z/11/Z)
European Commission (290123)
Medical Research Council (MR/R009791/1)
Identifiers
External DOI: https://doi.org/10.1101/gad.320069.118
This record's URL: https://www.repository.cam.ac.uk/handle/1810/288175
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