Show simple item record

dc.contributor.authorHardwick, Lauraen
dc.contributor.authorPhilpott, Annaen
dc.date.accessioned2019-01-18T15:14:29Z
dc.date.available2019-01-18T15:14:29Z
dc.identifier.issn2398-502X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/288234
dc.description.abstractProneural basic-helix-loop-helix (bHLH) proteins, such as Neurogenin2 (Ngn2) and Ascl1, are critical regulators at the onset of neuronal differentiation. Endogenously they have largely complementary expression patterns, and have conserved roles in the specification of distinct neuronal subtypes. In Xenopus embryos, xNgn2 is the master regulator of primary neurogenesis forming sensory, inter- and motor neurons within the neural plate, while xAscl1 is the master regulator of autonomic neurogenesis, forming noradrenergic neurons in the antero-ventral region of the embryo. Here we characterise neuronal subtype identity of neurons induced by xNgn2 in the ectoderm of whole Xenopus embryos in comparison with xAscl1, and in ectodermal “animal cap” explants. We find that the transcriptional cascades mediating primary and autonomic neuron formation are distinct, and while xNgn2 and xAscl1 can upregulate genes associated with a non-endogenous cascade, this expression is spatially restricted within the embryo. xNgn2 is more potent than xAscl1 at inducing primary neurogenesis as assayed by neural-β-tubulin. In ectoderm of the intact embryo, these induced primary neurons have sensory characteristics with no upregulation of motor neuron markers. In contrast, xNgn2 is able to up-regulate both sensory and motor neuron markers in naïve ectoderm of animal cap explants, suggesting a non-permissive environment for motor identity in the patterned ectoderm of the whole embryo.
dc.description.sponsorshipThis work is supported by the Wellcome Trust [203151] (AP). LH has been supported by an MRC Doctoral Training Award and Peterhouse Research Fellowship.
dc.publisherWellcome Open Research
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectStemCellInstituteen
dc.subjectNeurogenin2en
dc.subjectneurogenesisen
dc.subjectXenopusen
dc.subjectbHLHen
dc.subjectproneuralen
dc.subjectsensoryen
dc.subjectmotoren
dc.titlexNgn2 induces expression of predominantly sensory neuron markers in Xenopus whole embryo ectoderm but induces mixed subtype expression in isolated ectoderm explantsen
dc.typeArticle
prism.issueIdentifier144en
prism.publicationNameWellcome Open Researchen
prism.volume3en
dc.identifier.doi10.17863/CAM.33670
dcterms.dateAccepted2018-11-08en
rioxxterms.versionofrecord10.12688/wellcomeopenres.14911.1en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2018-11-08en
dc.contributor.orcidPhilpott, Anna [0000-0003-3789-2463]
dc.identifier.eissn2398-502X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (203151/Z/16/Z)
pubs.funder-project-idMRC (MC_PC_12009)
cam.issuedOnline2018-11-08en
dc.identifier.urlhttps://wellcomeopenresearch.org/articles/3-144/v1en


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International