Kinetic and thermodynamic effects of phosphorylation on p53 binding to MDM2.
Neira, José L
Johnson, Christopher M
Verma, Chandra S
Nature Publishing Group
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Yadahalli, S., Neira, J. L., Johnson, C. M., Tan, Y. S., Rowling, P., Chattopadhyay, A., Verma, C. S., & et al. (2019). Kinetic and thermodynamic effects of phosphorylation on p53 binding to MDM2.. Scientific reports, 9 (1), 693. https://doi.org/10.1038/s41598-018-36589-5
p53 is frequently mutated in human cancers. Its levels are tightly regulated by the E3 ubiquitin ligase MDM2. The complex between MDM2 and p53 is largely formed by the interaction between the N-terminal domain of MDM2 and the N-terminal transactivation (TA) domain of p53 (residues 15-29). We investigated the kinetic and thermodynamic basis of the MDM2/p53 interaction by using wild-type and mutant variants of the TA domain. We focus on the effects of phosphorylation at positions Thr18 and Ser20 including their substitution with phosphomimetics. Conformational propensities of the isolated peptides were investigated using in silico methods and experimentally by circular dichroism and 1H-NMR in aqueous solution. Both experimental and computational analyses indicate that the p53 peptides are mainly disordered in aqueous solution, with evidence of nascent helix around the Ser20-Leu25 region. Both phosphorylation and the phosphomimetics at Thr18 resulted in a decrease in the binding affinity by ten- to twenty-fold when compared to the wild-type. Phosphorylation and phosphomimetics at Ser20 resulted in a smaller decrease in the affinity. Mutation of residues Lys24 and Leu25 also disrupted the interaction. Our results may be useful for further development of peptide-based drugs targeting the MDM2/p53 interaction.
Humans, Threonine, Serine, Peptide Fragments, Spectrometry, Fluorescence, Circular Dichroism, Magnetic Resonance Spectroscopy, Binding Sites, Protein Conformation, Phosphorylation, Kinetics, Mutation, Thermodynamics, Tumor Suppressor Protein p53, Proto-Oncogene Proteins c-mdm2, Protein Interaction Domains and Motifs, Molecular Dynamics Simulation
Generalitat Valenciana BEST short-stay fellowship in the Department of Pharmacology, Cambridge, UK. Medical Research Foundation
External DOI: https://doi.org/10.1038/s41598-018-36589-5
This record's URL: https://www.repository.cam.ac.uk/handle/1810/288489
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/