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Transcriptional regulation of the genes involved in protein metabolism and processing in Saccharomyces cerevisiae.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Nightingale, Daniel JH 
Lilley, Kathryn S 
Oliver, Stephen G 

Abstract

Topological analysis of large networks, which focus on a specific biological process or on related biological processes, where functional coherence exists among the interacting members, may provide a wealth of insight into cellular functionality. This work presents an unbiased systems approach to analyze genetic, transcriptional regulatory and physical interaction networks of yeast genes possessing such functional coherence to gain novel biological insight. The present analysis identified only a few transcriptional regulators amongst a large gene cohort associated with the protein metabolism and processing in yeast. These transcription factors are not functionally required for the maintenance of these tasks in growing cells. Rather, they are involved in rewiring gene transcription in response to such major challenges as starvation, hypoxia, DNA damage, heat shock or the accumulation of unfolded proteins. Indeed, only a subset of these proteins were captured empirically in the nuclear-enriched fraction of non-stressed yeast cells, suggesting that the transcriptional regulation of protein metabolism and processing in yeast is primarily concerned with maintaining cellular robustness in the face of threat by either internal or external stressors.

Description

Keywords

network analysis, protein metabolic process, protein processing, proteomics, transcriptional regulation, yeast, Gene Expression Regulation, Fungal, Gene Regulatory Networks, Protein Processing, Post-Translational, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic

Journal Title

FEMS Yeast Res

Conference Name

Journal ISSN

1567-1356
1567-1364

Volume Title

19

Publisher

Oxford University Press (OUP)
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/K011138/1)
European Commission (289126)
Isaac Newton Trust (16.08(am))
Leverhulme Trust (ECF-2016-681)
BBSRC (1125103)
Biotechnology and Biological Sciences Research Council (BB/I016147/1)