TaDa! Analysing cell type-specific chromatin in vivo with Targeted DamID.
Current opinion in neurobiology
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Van Den Ameele, J., Krautz, R., & Brand, A. (2019). TaDa! Analysing cell type-specific chromatin in vivo with Targeted DamID.. Current opinion in neurobiology, 56 160-166. https://doi.org/10.1016/j.conb.2019.01.021
The emergence of neuronal diversity during development of the nervous system relies on dynamic changes in the epigenetic landscape of neural stem cells and their progeny. Targeted DamID (TaDa) is proving invaluable in identifying the genome-wide binding sites of chromatin-associated proteins in vivo, without fixation, cell isolation, or immunoprecipitation. The simplicity and efficiency of the technique has led to an ever-expanding TaDa toolbox. These tools enable profiling of gene expression and chromatin accessibility, as well as the identification of the genome-wide binding sites of chromatin complexes, transcription factors and RNAs. Here, we review these new developments, with particular emphasis on the use of TaDa in studying neuronal specification.
Chromatin, Transcription Factors, DNA Methylation, Binding Sites, Genome
WELLCOME TRUST (103792/Z/14/Z)
WELLCOME TRUST (105839/Z/14/Z)
Royal Society (RP150061)
Wellcome Trust (092096/Z/10/Z)
Cancer Research UK (A14492)
External DOI: https://doi.org/10.1016/j.conb.2019.01.021
This record's URL: https://www.repository.cam.ac.uk/handle/1810/289263