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dc.contributor.authorZarkan, Ashraf
dc.contributor.authorCaño-Muñiz, Santiago
dc.contributor.authorZhu, Jinbo
dc.contributor.authorAl Nahas, Kareem
dc.contributor.authorCama, Jehangir
dc.contributor.authorKeyser, Ulrich F
dc.contributor.authorSummers, David K
dc.date.accessioned2019-02-20T00:30:06Z
dc.date.available2019-02-20T00:30:06Z
dc.date.issued2019-03-07
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/289659
dc.description.abstractBacterial cells are critically dependent upon pH regulation. Here we demonstrate that indole plays a critical role in the regulation of the cytoplasmic pH of Escherichia coli. Indole is an aromatic molecule with diverse signalling roles. Two modes of indole signalling have been described: persistent and pulse signalling. The latter is illustrated by the brief but intense elevation of intracellular indole during stationary phase entry. We show that under conditions permitting indole production, cells maintain their cytoplasmic pH at 7.2. In contrast, under conditions where no indole is produced, the cytoplasmic pH is near 7.8. We demonstrate that pH regulation results from pulse, rather than persistent, indole signalling. Furthermore, we illustrate that the relevant property of indole in this context is its ability to conduct protons across the cytoplasmic membrane. Additionally, we show that the effect of the indole pulse that occurs normally during stationary phase entry in rich medium remains as a "memory" to maintain the cytoplasmic pH until entry into the next stationary phase. The indole-mediated reduction in cytoplasmic pH may explain why indole provides E. coli with a degree of protection against stresses, including some bactericidal antibiotics.
dc.description.sponsorshipThe Leverhulme Trust
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCell Membrane
dc.subjectCytoplasm
dc.subjectEscherichia coli
dc.subjectIndoles
dc.subjectPhosphatidylcholines
dc.subjectCulture Media
dc.subjectFlow Cytometry
dc.subjectSpectrometry, Fluorescence
dc.subjectSignal Transduction
dc.subjectCell Proliferation
dc.subjectPeriodicity
dc.subjectHydrogen-Ion Concentration
dc.subjectPhotons
dc.subjectUnilamellar Liposomes
dc.titleIndole Pulse Signalling Regulates the Cytoplasmic pH of E. coli in a Memory-Like Manner.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2019
prism.publicationNameSci Rep
prism.startingPage3868
prism.volume9
dc.identifier.doi10.17863/CAM.36907
dcterms.dateAccepted2019-02-08
rioxxterms.versionofrecord10.1038/s41598-019-40560-3
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-03-07
dc.contributor.orcidZarkan, Ashraf [0000-0003-3582-124X]
dc.contributor.orcidAl Nahas, Kareem [0000-0003-4568-5894]
dc.contributor.orcidCama, Jehangir [0000-0002-5982-1941]
dc.contributor.orcidKeyser, Ulrich F [0000-0003-3188-5414]
dc.contributor.orcidSummers, David K [0000-0002-8243-9521]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
pubs.funder-project-idLeverhulme Trust (RPG-2015-184)
pubs.funder-project-idEuropean Research Council (647144)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/M008258/1)
pubs.funder-project-idEuropean Research Council (261101)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/F002912/1)
cam.issuedOnline2019-03-07
cam.orpheus.successThu Jan 30 10:51:40 GMT 2020 - The item has an open VoR version.
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International