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dc.contributor.authorZarkan, Ashrafen
dc.contributor.authorCaño-Muñiz, Santiagoen
dc.contributor.authorZhu, Jinboen
dc.contributor.authorAl Nahas, Kareemen
dc.contributor.authorCama, Jehangiren
dc.contributor.authorKeyser, Ulrichen
dc.contributor.authorSummers, Daviden
dc.date.accessioned2019-02-20T00:30:06Z
dc.date.available2019-02-20T00:30:06Z
dc.date.issued2019-03-07en
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/289659
dc.description.abstractBacterial cells are critically dependent upon pH regulation. Here we demonstrate that indole plays a critical role in the regulation of the cytoplasmic pH of Escherichia coli. Indole is an aromatic molecule with diverse signalling roles. Two modes of indole signalling have been described: persistent and pulse signalling. The latter is illustrated by the brief but intense elevation of intracellular indole during stationary phase entry. We show that under conditions permitting indole production, cells maintain their cytoplasmic pH at 7.2. In contrast, under conditions where no indole is produced, the cytoplasmic pH is near 7.8. We demonstrate that pH regulation results from pulse, rather than persistent, indole signalling. Furthermore, we illustrate that the relevant property of indole in this context is its ability to conduct protons across the cytoplasmic membrane. Additionally, we show that the effect of the indole pulse that occurs normally during stationary phase entry in rich medium remains as a “memory” to maintain the cytoplasmic pH until entry into the next stationary phase. The indole-mediated reduction in cytoplasmic pH may explain why indole provides E. coli with a degree of protection against stresses, including some bactericidal antibiotics.
dc.description.sponsorshipThe Leverhulme Trust
dc.format.mediumElectronicen
dc.languageengen
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCell Membraneen
dc.subjectCytoplasmen
dc.subjectEscherichia colien
dc.subjectIndolesen
dc.subjectPhosphatidylcholinesen
dc.subjectCulture Mediaen
dc.subjectFlow Cytometryen
dc.subjectSpectrometry, Fluorescenceen
dc.subjectSignal Transductionen
dc.subjectCell Proliferationen
dc.subjectPeriodicityen
dc.subjectHydrogen-Ion Concentrationen
dc.subjectPhotonsen
dc.subjectUnilamellar Liposomesen
dc.titleIndole Pulse Signalling Regulates the Cytoplasmic pH of E. coli in a Memory-Like Manner.en
dc.typeArticle
prism.issueIdentifier1en
prism.publicationDate2019en
prism.publicationNameScientific reportsen
prism.startingPage3868
prism.volume9en
dc.identifier.doi10.17863/CAM.36907
dcterms.dateAccepted2019-02-08en
rioxxterms.versionofrecord10.1038/s41598-019-40560-3en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-03-07en
dc.contributor.orcidZarkan, Ashraf [0000-0003-3582-124X]
dc.contributor.orcidZhu, Jinbo [0000-0003-4935-825X]
dc.contributor.orcidAl Nahas, Kareem [0000-0003-4568-5894]
dc.contributor.orcidCama, Jehangir [0000-0002-5982-1941]
dc.contributor.orcidKeyser, Ulrich [0000-0003-3188-5414]
dc.contributor.orcidSummers, David [0000-0002-8243-9521]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idLeverhulme Trust (RPG-2015-184)
pubs.funder-project-idECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (647144)
pubs.funder-project-idEPSRC (EP/M008258/1)
pubs.funder-project-idEuropean Research Council (261101)
cam.orpheus.successThu Jan 30 10:51:40 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International