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dc.contributor.authorShishmarev, Dmitry
dc.contributor.authorKuchel, Philip W
dc.contributor.authorPagès, Guilhem
dc.contributor.authorWright, Alan J
dc.contributor.authorHesketh, Richard L
dc.contributor.authorKreis, Felix
dc.contributor.authorBrindle, Kevin M
dc.date.accessioned2019-02-26T00:30:10Z
dc.date.available2019-02-26T00:30:10Z
dc.date.issued2018
dc.identifier.issn2399-3642
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/289954
dc.description.abstractMethylglyoxal is a faulty metabolite. It is a ubiquitous by-product of glucose and amino acid metabolism that spontaneously reacts with proximal amino groups in proteins and nucleic acids, leading to impairment of their function. The glyoxalase pathway evolved early in phylogeny to bring about rapid catabolism of methylglyoxal, and an understanding of the role of methylglyoxal and the glyoxalases in many diseases is beginning to emerge. Metabolic processing of methylglyoxal is very rapid in vivo and thus notoriously difficult to detect and quantify. Here we show that 13C nuclei in labeled methylglyoxal can be hyperpolarized using dynamic nuclear polarization, providing 13C nuclear magnetic resonance signal enhancements in the solution state close to 5,000-fold. We demonstrate the applications of this probe of metabolism for kinetic characterization of the glyoxalase system in isolated cells as well as mouse brain, liver and lymphoma in vivo.
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleGlyoxalase activity in human erythrocytes and mouse lymphoma, liver and brain probed with hyperpolarized 13C-methylglyoxal.
dc.typeArticle
prism.publicationDate2018
prism.publicationNameCommun Biol
prism.startingPage232
prism.volume1
dc.identifier.doi10.17863/CAM.37184
dcterms.dateAccepted2018-11-29
rioxxterms.versionofrecord10.1038/s42003-018-0241-1
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-01
dc.contributor.orcidShishmarev, Dmitry [0000-0002-0724-0002]
dc.contributor.orcidPagès, Guilhem [0000-0001-9368-5237]
dc.contributor.orcidWright, Alan J [0000-0002-4577-5681]
dc.contributor.orcidKreis, Felix [0000-0003-3340-3861]
dc.contributor.orcidBrindle, Kevin M [0000-0003-3883-6287]
dc.identifier.eissn2399-3642
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (642773)
cam.issuedOnline2018-12-21


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International