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Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals.

Accepted version
Peer-reviewed

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Article

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Authors

Loncaric, Igor 
Kübber-Heiss, Anna 
Posautz, Annika 
Ruppitsch, Werner 
Lepuschitz, Sarah 

Abstract

The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecR1-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.

Description

Keywords

Animals, Antimicrobial resistance, Coagulase-negative Staphylococcus spp., mecA, mecC, Animals, Animals, Domestic, Animals, Wild, Anti-Bacterial Agents, Bacterial Proteins, Cefoxitin, Coagulase, DNA, Bacterial, Drug Resistance, Multiple, Bacterial, Goats, Lynx, Methicillin Resistance, Microbial Sensitivity Tests, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Sheep, Staphylococcus

Journal Title

Vet Microbiol

Conference Name

Journal ISSN

0378-1135
1873-2542

Volume Title

230

Publisher

Elsevier BV
Sponsorship
Medical Research Council (G1001787)
Medical Research Council (MR/N002660/1)
Medical Research Council (MR/P007201/1)