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SRSF1-dependent nuclear export inhibition of C9ORF72 repeat transcripts prevents neurodegeneration and associated motor deficits.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Hautbergue, Guillaume M 
Castelli, Lydia M 
Ferraiuolo, Laura 
Sanchez-Martinez, Alvaro 
Cooper-Knock, Johnathan 

Abstract

Hexanucleotide repeat expansions in the C9ORF72 gene are the commonest known genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia. Expression of repeat transcripts and dipeptide repeat proteins trigger multiple mechanisms of neurotoxicity. How repeat transcripts get exported from the nucleus is unknown. Here, we show that depletion of the nuclear export adaptor SRSF1 prevents neurodegeneration and locomotor deficits in a Drosophila model of C9ORF72-related disease. This intervention suppresses cell death of patient-derived motor neuron and astrocytic-mediated neurotoxicity in co-culture assays. We further demonstrate that either depleting SRSF1 or preventing its interaction with NXF1 specifically inhibits the nuclear export of pathological C9ORF72 transcripts, the production of dipeptide-repeat proteins and alleviates neurotoxicity in Drosophila, patient-derived neurons and neuronal cell models. Taken together, we show that repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats and reveal a novel promising therapeutic target for neuroprotection.

Description

Keywords

Adult, Aged, Amyotrophic Lateral Sclerosis, Animals, Astrocytes, C9orf72 Protein, Cell Line, Coculture Techniques, Disease Models, Animal, Drosophila, Female, Frontotemporal Dementia, Humans, Male, Mice, Middle Aged, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, RNA-Binding Proteins, Rats, Serine-Arginine Splicing Factors, Transcription Factors

Journal Title

Nature Communications

Conference Name

Journal ISSN

2041-1723
2041-1723

Volume Title

8

Publisher

Nature Publishing Group
Sponsorship
Medical Research Council (MC_UU_00015/6)
Medical Research Council (MC_UP_1501/1)
European Research Council (309742)
Medical Research Council (MC_UU_00015/7)
MRC, ERC, FP7