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dc.contributor.authorSalesse-Smith, Coralie E
dc.contributor.authorSharwood, Robert E
dc.contributor.authorBusch, Florian A
dc.contributor.authorKromdijk, Johannes
dc.contributor.authorBardal, Viktoriya
dc.contributor.authorStern, David B
dc.date.accessioned2019-04-04T09:40:12Z
dc.date.available2019-04-04T09:40:12Z
dc.date.issued2018-10
dc.identifier.issn2055-026X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/291118
dc.description.abstractRubisco catalyses a rate-limiting step in photosynthesis and has long been a target for improvement due to its slow turnover rate. An alternative to modifying catalytic properties of Rubisco is to increase its abundance within C4 plant chloroplasts, which might increase activity and confer a higher carbon assimilation rate. Here, we overexpress the Rubisco large (LS) and small (SS) subunits with the Rubisco assembly chaperone RUBISCO ASSEMBLY FACTOR 1 (RAF1). While overexpression of LS and/or SS had no discernable impact on Rubisco content, addition of RAF1 overexpression resulted in a >30% increase in Rubisco content. Gas exchange showed a 15% increase in CO2 assimilation (ASAT) in UBI-LSSS-RAF1 transgenic plants, which correlated with increased fresh weight and in vitro Vcmax calculations. The divergence of Rubisco content and assimilation could be accounted for by the Rubisco activation state, which decreased up to 23%, suggesting that Rubisco activase may be limiting Vcmax, and impinging on the realization of photosynthetic potential from increased Rubisco content.
dc.description.sponsorshipThis research was supported by the Agriculture and Food Research Initiative from the National Institute of Food and Agriculture, US Department of Agriculture, under award number 2016-67013-24464. Travel to the Australian National University was supported by the Mario Einaudi Center for International Studies, International Research Travel Grant at Cornell University.
dc.languageeng
dc.publisherPalgrave Macmillan Ltd.
dc.titleOverexpression of Rubisco subunits with RAF1 increases Rubisco content in maize.
dc.typeArticle
prism.endingPage810
prism.issueIdentifier10
prism.publicationDate2018
prism.publicationNameNature Plants
prism.startingPage802
prism.volume4
dc.identifier.doi10.17863/CAM.38299
dcterms.dateAccepted2018-08-15
rioxxterms.versionofrecord10.1038/s41477-018-0252-4
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-10
dc.contributor.orcidKromdijk, Johannes [0000-0003-4423-4100]
dc.identifier.eissn2055-0278
dc.publisher.urlhttps://www.nature.com/articles/s41477-018-0252-4#article-info
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-10-01
dc.identifier.urlhttps://www.nature.com/articles/s41477-018-0252-4#article-info
rioxxterms.freetoread.startdate2019-04-01


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