Hidden in plain sight: what remains to be discovered in the eukaryotic proteome?
The Royal Society
MetadataShow full item record
Wood, V., Lock, A., Harris, M., Rutherford, K., Bähler, J., & Oliver, S. (2019). Hidden in plain sight: what remains to be discovered in the eukaryotic proteome?. Open biology, 9 (2), 180241. https://doi.org/10.1098/rsob.180241
The first decade of genome sequencing stimulated an explosion in the characterization of unknown proteins. More recently, the pace of functional discovery has slowed, leaving around 20% of the proteins even in well-studied model organisms without informative descriptions of their biological roles. Remarkably, many uncharacterized proteins are conserved from yeasts to human, suggesting that they contribute to fundamental biological processes. To fully understand biological systems in health and disease, we need to account for every part of the system. Unstudied proteins thus represent a collective blind spot that limits the progress of both basic and applied biosciences. We use a simple yet powerful metric based on Gene Ontology (GO) biological process terms to define characterized and uncharacterized proteins for human, budding yeast, and fission yeast. We then identify a set of conserved but unstudied proteins in S. pombe , and classify them based on a combination of orthogonal attributes determined by large-scale experimental and comparative methods. Finally, we explore possible reasons why these proteins remain neglected and propose courses of action to raise their profile and thereby reap the benefits of completing the catalog of proteins' biological roles.
Eukaryotic Cells, Humans, Saccharomyces cerevisiae, Schizosaccharomyces, Saccharomyces cerevisiae Proteins, Schizosaccharomyces pombe Proteins, Proteome, Gene Expression Profiling, Proteomics, Species Specificity, Gene Ontology
WELLCOME TRUST (104967/Z/14/Z)
External DOI: https://doi.org/10.1098/rsob.180241
This record's URL: https://www.repository.cam.ac.uk/handle/1810/291162
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
Recommended or similar items
The following licence files are associated with this item: