The dataset contains experimental and simulation data on the interaction of gold nanoparticles (NPs) and DNA-functionalised liposomes as described in the paper "Kinetics of Nanoparticle-Membrane Adhesion Mediated by Multivalent Interactions”. Experimental raw data are divided into two compressed folders relative to DLS and UV-vis experiments, and named accordingly. Simulation data are in a separate compressed folder (SimulationData).

The “DLS” folder contains sub-folders labelled “LUVXXX”, where XXX indicates the number of receptors tethered on each liposome in the system, accordingly to Table 1 in the main text of the paper (370, 770, 1190, 1640). Each folder contains tab-separated text files labelled either as “Data_ratio*.txt” or “Time_ratio*.txt”, where “” indicates the ratio between the number of NPs and LUVs in the system, as reported in Table 1 of the main text (7, 70, 290). “Data_ratio.txt” is a multi-column tab-separated file containing the raw DLS autocorrelation functions. The first column contains the delay times (“tau”, see associated publication), while in the other columns (from the second to the last) contain the autocorrelation values acquired at different times “t” (see associated publication) from the beginning of the experiment. The file “Time_ratio*.txt” is a single column file containing the list of times “t” associated to each autocorrelation function of the corresponding “Data_ratio*.txt”. These data have been used to produce figures 3, 4a, 4b, 6a, 6b and 7 as described in the associated publication. The folder named “Controls” contains, in the same format, data associated to control experiments used to produce figure S5.1 in the Supporting Information.

The “UV-vis” folder contains raw data underlying figure S3.1 of the Supporting Information. Each multi-column tab-separated text file is labelled as “Melting_sampleY”, where Y=1, 2, 3 correspond to the three data points in figure S3.1, with increasing rho_R-LUV (horizontal axis in the figure). Each file contains two or three sets of data in a four-column format. These different sets correspond to repetitions of the same experiment. In each 4-column set, the first and the third column contain temperature (in degrees Celsius), column 2 contains the absorbance data measured at the plasmon-resonance frequency of free NPs at the temperatures reported in column 1, while column 4 contains the absorbance data recorded at the plasmon-resonance frequency of NP-LUV complexes at the temperatures reported in column 3.

The “SimulationData” folder contains 4 other folders, each corresponding to the different figures and panels featuring simulation data: FIGURE_4C, FIGURE_5A, FIGURE_5B and FIGURE_6A. All folders contain multiple .txt files, each corresponding to the different curves in the figures. All files contain two space-separated columns corresponding to the quantities on the horizontal and vertical axes of the figures.

FIGURE_4C contains simulated data for the NP-LUV adhesion time as a function of the receptor surface density for three different values of the NP-LUV concentration ratio (7, 70, 290).

FIGURE_5A contains simulated data for the number of NP adhering on the LUV as a function of time for 5 values of the simulation on-rate (1E-3, 1E-2, 1E-1, 1E5, infinity). Time is expressed in seconds.

FIGURE_5B contains the average number of ligand-receptor bonds per NP VS time for the same values of the on-rate of FIGURE_5A.

FIGURE_6A contains the simulated steady-state fraction of adhering NPs as a function of the receptor surface density for three different values of the NP-LUV concentration ratio (7, 70, 290).