Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Guo, Zhiang https://orcid.org/0000-0003-3620-9554
Segal, Marisa https://orcid.org/0000-0003-1848-9388
Abstract
Mitotic exit is determined by multiple spatial and temporal cues from the spindle poles and the two compartments in a dividing yeast cell-the mother and the bud. These signals are ultimately integrated by the activation of the mitotic exit network (MEN) to promote persistent release of Cdc14 from the nucleolus. Live imaging analysis using fluorescent protein tags is invaluable to dissect this critical decision-making trigger. Here, we present protocols for routine yeast live cell microscopy applicable to this problem.
Description
Title
Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.
Keywords
Cell population analysis, Digital image, Fluorescence microscopy, Fluorescent protein tags, Live cell imaging, Single cell analysis, Still imaging, Time lapse, Anaphase, Cell Cycle Proteins, Green Fluorescent Proteins, Image Processing, Computer-Assisted, Microscopy, Fluorescence, Mitosis, Optical Imaging, Protein Serine-Threonine Kinases, Protein Tyrosine Phosphatases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Single-Cell Analysis
Is Part Of
Methods in Molecular Biology
Book type
Publisher
Springer New York
Publisher DOI
ISBN
978-1-4939-6500-7
Rights
All rights reserved