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Robust, universal biomarker assay to detect senescent cells in biological specimens.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Evangelou, Konstantinos 
Lougiakis, Nikolaos 
Rizou, Sophia V 
Kotsinas, Athanassios 
Kletsas, Dimitris 

Abstract

Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.

Description

Keywords

Sudan Black B, aging, biotin‐linked compounds, immunohistochemistry, senescence, Animals, Biological Assay, Biomarkers, Biotin, Cell Line, Tumor, Cellular Senescence, Humans, Mice

Journal Title

Aging Cell

Conference Name

Journal ISSN

1474-9718
1474-9726

Volume Title

16

Publisher

Wiley