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dc.contributor.authorStröhl, Florianen
dc.contributor.authorKaminski, Clemensen
dc.date.accessioned2019-04-16T23:31:54Z
dc.date.available2019-04-16T23:31:54Z
dc.date.issued2019-04-23en
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/291771
dc.description.abstractThe deconvolution of widefield fluorescence images provides only guesses of spatial frequency information along the optical axis due to the so called missing cone in the optical transfer function. Retaining the single-shot imaging speed of deconvolution microscopy while gaining access to missing cone information is thus highly desirable for microscopy of volumetric samples. Here, we present a concept that superimposes two orthogonally polarized excitation lattices with a phase-shift of p between them. In conjunction with a non-iterative image reconstruction algorithm this permits the restoration of missing cone information. We show how fluorescence anisotropy could be used as a method to encode and decode the patterns simultaneously and develop a rigorous theoretical framework for the method. Through in-silico experiments and imaging of fixed biological cells on a structured illumination microscope that emulates the proposed setup we validate the feasibility of the method.
dc.format.mediumElectronicen
dc.languageengen
dc.publisherNature Publishing Group
dc.rightsAll rights reserved
dc.titleA concept for single-shot volumetric fluorescence imaging via orthogonally polarized excitation lattices.en
dc.typeArticle
prism.issueIdentifier1en
prism.publicationDate2019en
prism.publicationNameScientific reportsen
prism.startingPage6425
prism.volume9en
dc.identifier.doi10.17863/CAM.38931
dcterms.dateAccepted2019-04-08en
rioxxterms.versionofrecord10.1038/s41598-019-42743-4en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-04-23en
dc.contributor.orcidStröhl, Florian [0000-0002-2603-0780]
dc.contributor.orcidKaminski, Clemens [0000-0002-5194-0962]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (EP/H018301/1)
pubs.funder-project-idMRC (MR/K02292X/1)
pubs.funder-project-idWellcome Trust (203249/Z/16/Z)
pubs.funder-project-idWellcome Trust (089703/Z/09/Z)
pubs.funder-project-idMRC (MR/K015850/1)
cam.orpheus.successMon Jun 08 08:19:56 BST 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2022-04-16


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