Extracellular Interaction Screening Using CRISPR Activation
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Authors
Chong, Zheng Shan
Abstract
Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo myelin-associated inhibitory proteins are ligands for ADGRB1. This method will enable extracellular receptor-ligand identification on a genome-wide scale.
Description
Date
2018-09-28
Advisors
Wright, Gavin
Keywords
CRISPR, protein-protein interactions, high-throughput screening
Qualification
Doctor of Philosophy (PhD)
Awarding Institution
University of Cambridge