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Extracellular Interaction Screening Using CRISPR Activation


Type

Thesis

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Authors

Chong, Zheng Shan 

Abstract

Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo myelin-associated inhibitory proteins are ligands for ADGRB1. This method will enable extracellular receptor-ligand identification on a genome-wide scale.

Description

Date

2018-09-28

Advisors

Wright, Gavin

Keywords

CRISPR, protein-protein interactions, high-throughput screening

Qualification

Doctor of Philosophy (PhD)

Awarding Institution

University of Cambridge