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dc.contributor.authorFerro, Magali P
dc.contributor.authorLeclerc, Lara
dc.contributor.authorSleiman, Mohamad
dc.contributor.authorMarchiori, Bastien
dc.contributor.authorPourchez, Jérémie
dc.contributor.authorOwens, Roisin
dc.contributor.authorRamuz, Marc
dc.date.accessioned2019-05-02T23:30:58Z
dc.date.available2019-05-02T23:30:58Z
dc.date.issued2019-03
dc.identifier.issn2366-7478
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/292265
dc.description.abstractE-cigarettes have been suggested as a potentially healthier alternative to cigarettes based on studies using cell viability, DNA damage, and transcriptional response assays. However, little is known about the effect of e-cigarette aerosols on the integrity of the tracheal epithelium, specifically with respect to barrier resistance. This is partly due to the lack of methods for monitoring epithelia at the air-liquid interface (ALI), i.e., under physiological conditions. Here, it is shown that an organic electrochemical transistor can be adapted for the measurement of barrier resistance at the ALI. This technology enables accurate, continuous quantification of tracheal barrier integrity through the use of a conformable gate electrode placed on top of the cell-secreted mucus, obviating the need for addition of culture medium or buffer as a conductance medium for rigid electrodes. This platform allows for the detection of a dose-dependent, rapid decrease in barrier resistance of an in vitro model of human bronchial epithelium (MucilAir) after E-cigarette aerosols exposure. The system represents a powerful tool to study tissue responses of the human airway epithelium to inhaled smoke. The same technology will have broad applications for toxicology studies on other tissues with ALI, including other airway tissues and skin.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherWiley
dc.rightsAll rights reserved
dc.subjectTrachea
dc.subjectCells, Cultured
dc.subjectHumans
dc.subjectCell Culture Techniques
dc.subjectEquipment Design
dc.subjectTobacco Smoke Pollution
dc.subjectModels, Biological
dc.subjectMiddle Aged
dc.subjectElectrochemical Techniques
dc.subjectElectronic Nicotine Delivery Systems
dc.titleEffect of E Cigarette Emissions on Tracheal Cells Monitored at the Air-Liquid Interface Using an Organic Electrochemical Transistor.
dc.typeArticle
prism.issueIdentifier3
prism.publicationDate2019
prism.publicationNameAdv Biosyst
prism.startingPagee1800249
prism.volume3
dc.identifier.doi10.17863/CAM.39414
dcterms.dateAccepted2018-11-22
rioxxterms.versionofrecord10.1002/adbi.201800249
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-03
dc.contributor.orcidOwens, Roisin [0000-0001-7856-2108]
dc.identifier.eissn2366-7478
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-12-21
rioxxterms.freetoread.startdate2020-03-01


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